含量测定和指纹图谱结合LC-MS技术整体评价银杏叶片的质量  

Quantitative and qualitative evaluation on tablets of Ginkgo biloba leaves using fingerprint and LC-MS analysis

作　　者：梁文琳 谢达温[1] 丁岗[1] 徐殿红[1] 孙永成[1] 廉源沛[1] 李艳静[1] 萧伟[1] 

机构地区：[1]江苏康缘药业股份有限公司,江苏连云港222001 [2]中国药科大学,江苏南京210009

出　　处：《中国中药杂志》2015年第9期1738-1743,共6页China Journal of Chinese Materia Medica

基　　金：国家科技支撑计划项目(2011BA04B03)

摘　　要：建立银杏叶片合理质量控制的方法，评价市售不同厂家银杏叶片的质量。收集市售银杏叶片，采用HPLC对其进行总黄酮醇苷和萜类内酯含量测定，根据2010年版《中国药典》一部方法进行测定，2批银杏叶片总黄酮醇苷含量不合格，3批银杏叶片萜类内酯含量不合格；建立HPLC指纹图谱检测方法对其进行检测，不同厂家生产的16批银杏叶片HPLC指纹图谱标记出31个共有峰，与生成的对照指纹图谱相似度为0．763～0．989；采用LC-MS对指纹图谱色谱峰进行指认，鉴定出31个黄酮类成分。以总黄酮醇苷含量、萜类内酯含量和指纹图谱结合LC-MS技术整体评价银杏叶片的质量，为银杏叶片质量研究提供一些研究思路。A reasonable method for the quality control of tablets of Ginkgo biloba leaves was established in this paper. The total fla- vonol glycosides and terpene lactones of G. biloba tablets were quantified by HPLC. Totally, 16 batches of the commercially available tablets of G. biloba leaves were determined. Among of them, 2 batches were unqualified in the content of total flavonol glycosides, and 3 batches were unqualified in the content of terpene lactones. A validated HPLC fingerprint method was established to evaluate the com- mercially available tablets of G. biloba leaves with the assistance of LC-MS. Sixteen batches showed the similarity of 0. 763-0. 989. There were 31 fingerprint chromatogram peaks were identified as flavonoids compositions by LC-MS. This provides a research idea for the quality control of tablets of G. biloba leaves.

关 键 词：银杏叶片 银杏叶标准提取物 总黄酮醇苷 指纹图谱 LC-MS 质量评价 

分 类 号：R286.0[医药卫生—中药学] O657.63[医药卫生—中医学]