黄连总生物碱与小檗碱对嗜水气单胞菌细胞膜损伤机制的研究  

Study on membrane injury mechanism of total alkaloids and berberine from Coptidis Rhizoma on Aeromonas hydrophila

作　　者：薛东芳[1,2] 邹宗尧 陈彪[1,2] 王燕枝[3,2] 吴昊[1,2] 叶小利[1,2] 李学刚[3,2] 

机构地区：[1]西南大学生命科学学院,重庆400715 [2]重庆药物过程与质量控制工程技术研究中心,重庆400716 [3]西南大学药学院,重庆400716

出　　处：《中国中药杂志》2015年第9期1787-1792,共6页China Journal of Chinese Materia Medica

基　　金：国家"十二五"科技支撑计划项目(2011BAI13B02-1);重庆市教委重点项目(KJZH14203);教育部博士点基金(20130182110023);重庆市百名高端工程技术人才培养计划(2013-2016)

摘　　要：为探索黄连总生物碱及小檗碱对嗜水气单胞菌的抑菌活性及作用机制,测定了黄连总生物碱和小檗碱对嗜水气单胞菌的最小抑菌浓度、膜通透性、膜流动性、膜蛋白构象及毒力因子的影响。结果显示,黄连总生物碱和小檗碱对嗜水气单胞菌均有抑菌活性,其最小抑菌浓度分别为62.5,125 mg·L-1。黄连总生物碱和小檗碱能增加膜脂流动性,改变膜蛋白构象,并使菌体膜通透性分别增加24.52%,19.66%。此外,黄连总生物碱和小檗碱显著性地抑制菌体外毒素的溶血活性及aer A和hly A mRNA的表达(P<0.05,P<0.01),同时降低嗜水气单胞菌内毒素的分泌量和Lpx C mRNA的表达(P<0.05,P<0.01)。结果表明,黄连总生物碱和小檗碱对嗜水气单胞菌的抑菌作用可能与细胞膜损伤有关,通过改变膜脂流动性和膜蛋白构象使菌体膜通透性增加从而抑制了细菌生长;同时降低了细菌的毒力因子的分泌,使得菌体的致病性降低。To explore the antibacterial activity and mechanism of total alkaloids and berberine from Coptidis Rhizoma on Aeromonas hydrophila, and determine the effect of total alkaloids and berberine from Coptidis Rhizoma on minimum inhibitory concentrations, perme- ability and fluidity of cell membrane, conformation of membrane proteins and virulence factors of A. hydrophila. The results showed that both total alkaloids and berberine from Coptidis Rhizoma had antibacterial activities on A. hydrophila, with minimum inhibitory concentra- tions of 62. 5 and 125 mg · L^-1, respectively. Total alkaloids and berberine from Coptidis Rhizoma could increase the fluidity of mem- brane, change the conformation of membrane proteins and increase the permeability of bacteria membrane by 24. 52% and 19. 66%, re- spectively. Besides, total alkaloids and berberine from Coptidis Rhizoma significantly decreased the hemolysis of exotoxin and the mRNA expressions of aerA and hlyA （ P 〈 0. 05, P 〈 0. 01 ）, the secretion of endotoxin and the mRNA expression of LpxC （ P 〈 0. 05, P 〈 0. 01 ）. The results suggested that the antibacterial activity of total alkaloids and berberine from Coptidis Rhizoma on A. hydrophila may be related to the bacteria membrane injury. They inhibited the bacterial growth by increasing membrane lipid fluidity and changing confor-mation of membrane proteins, and reduced the secretion of virulence factors of A. hydrophila to weaken the pathogenicity.

关 键 词：黄连生物碱 嗜水气单胞菌 抑菌机制 

分 类 号：R285[医药卫生—中药学]