铁皮石斛花提取物对甲亢型阴虚小鼠的影响  

Effect of extracts from Dendrobii ifficinalis flos on hyperthyroidism Yin deficiency mice

作　　者：雷珊珊[1] 吕圭源[1,2] 金泽武 李波[2] 杨正标[2] 陈素红[1,2] 

机构地区：[1]温州医科大学,浙江温州325035 [2]浙江中医药大学,浙江杭州310053

出　　处：《中国中药杂志》2015年第9期1793-1797,共5页China Journal of Chinese Materia Medica

基　　金：浙江省重大科技项目(2006C13014);国家"十二五"科技支撑计划项目(2011BAI04B06);浙江省重点实验室项目(2012E10002);浙江省卫生高层次创新人才培养工程项目(浙卫发[2010]190号)

摘　　要：该文采用甲亢型阴虚模型小鼠,研究铁皮石斛花的滋阴功效并探索其作用机制,为铁皮石斛花资源的开发利用,减少资源浪费提供药理学依据。ICR小鼠分为正常对照组、模型对照组、阳性对照组(六味地黄丸)及铁皮石斛花提取物高、低剂量组(6,4 g·kg-1)。采用连续灌胃甲状腺素30 d制备甲亢型阴虚小鼠模型。边造模边给药,连续给药30 d。给药4周,测定小鼠体征(面温、痛阈、心率及自主活动),采用激光多普勒技术测定耳面部微循环血流量。末次给药后,禁食不禁水,眼眶取血分离血清,全自动生化分析仪检测血清AST(天冬氨酸氨基转移酶)、ALT(丙氨酸氨基转移酶)、TG(甘油三酯)及TP(总蛋白)水平;酶联免疫法检测血清T3(三碘甲状腺原氨酸)、T4(甲状腺素)及TSH(促甲状腺素)水平。结果显示,1与对照组相比,模型组小鼠耳面部微循环血流量、面温及心率显著升高(P<0.05,P<0.01),痛域显著降低(P<0.01);血清AST,ALT水平显著升高(P<0.01),TG水平显著降低(P<0.01);血清T3水平升高(P<0.05),TSH水平显著降低(P<0.01)。2与模型组比,铁皮石斛花提取物高剂量不仅能显著降低模型小鼠面部微循环血流量、面温及心率(P<0.05,P<0.01),还能显著升高小鼠痛域值(P<0.01)。铁皮石斛花提取物高剂量显著降低血清AST水平(P<0.05),升高血清TG水平(P<0.01);铁皮石斛花提取物低剂量能显著降低血清AST,ALT水平(P<0.01,P<0.05)。铁皮石斛花提取物高、低剂量均能显著降低模型小鼠血清T3水平、显著升高血清TSH水平(P<0.05)。铁皮石斛花不仅能改善甲亢型阴虚小鼠模型"颧红、心悸"之阴虚症状,还能改善甲状腺轴功能亢进导致的肝功能损伤,其作用机制可能是铁皮石斛花通过影响甲状腺素物质代谢,改善微循、减慢心率而产生滋阴护肝作用。Some unhealthy life habits, such as long-term smoking, heavy drinking, sexual overstrain and frequent stay-up could in- duce the Yin deficiency symptoms of zygomatic red and dysphoria. Stems of Dendrobii officinalis flos （DOF） showed the efficacy of nourishing Yin. In this study, the hyperthyroidism Yin deficiency model was set up to study the yin nourishing effect and action mecha- nism of DOF, in order to provide the pharmacological basis for developing DOF resources and decreasing resource wastes. ICR mice were divided into five groups： the normal control group, the model control group, the positive control group and DOF extract groups （6, 4 g · kg^-1 ）. Except for the normal group, the other groups were administrated with thyroxine for 30 d to set up the hyperthyroid- ism yin deficiency model. At the same time, the other groups were administrated with the corresponding drugs for 30 d. After adminis- tration for 4 weeks, the signs （facial temperature, pain domain, heart rate and autonomic activity） in mice were measured, and the fa- cial and ear micro-circulation blood flow were detected by laser Doppler technology. After the last administration, all mice were fasted for 12 hours, blood were collected from their orbits, and serum were separated to detect AST, ALT, TG and TP by the automatic bio- chemistry analyzer and test T3 , T4 and TSH levels by ELISA. ① Compared with the normal control group, the model control group showed significant increases in facial and ear micro-circulation blood flow, facial temperature and heart rate （P 〈 0. 05, P 〈 0. 01 ）, serum AST, ALT （P 〈 0. 01 ）, T3 level （ P 〈 0. 05 ）, TSH level （ P 〈 0. 05 ） and notable deceases in pain domain （ P 〈 0. 01 ）, TG level （P 〈0. 01 ）. ② Compared with the model control group, extracts from DOF （6 g · kg^-1 ） could notably reduce facial and ear mi- cro-circulation blood flow, facial temperature and heart rate （P 〈 0. 05, P 〈 0. 01 ） and AST （P 〈 0. 05 ） and enhance pain d

关 键 词：铁皮石斛花 阴虚 模型 小鼠 甲状腺素 滋阴 

分 类 号：R285.5[医药卫生—中药学]