大花杓兰内生真菌多样性研究  

Diversity of Endophytic Fungi in Cypripedium macranthum

作　　者：侯晓强[1] 付亚娟[1] 袁建平[1] 吴智艳[1] 王利冉 李微[1] 

机构地区：[1]廊坊师范学院生命科学学院/河北省高校食药用菌应用技术研发中心,河北廊坊065000

出　　处：《湖北农业科学》2015年第6期1357-1360,共4页Hubei Agricultural Sciences

基　　金：国家自然科学基金项目(31100314);河北省自然科学基金项目(C2012408007);河北省遗传学重点发展学科建设项目;廊坊师范学院微生物学重点学科项目

摘　　要：对大花杓兰(Cypripedium macranthum Sw.)内生真菌类群组成及多样性进行了分析。结果表明,从大花杓兰植株中分离到内生真菌59株,利用ITS-r DNA序列分子鉴定法将大花杓兰内生真菌鉴定为18个分类单元,分布在7纲9目11科16属。北京大花杓兰内生真菌包括12个属,内生真菌的多样性指数H=1.39、D=0.96,优势菌群为毛壳菌属(Chaetomium)和茎点霉属(Phoma)真菌,分别占总菌株数的15.25%和8.47%;吉林大花杓兰样品内生真菌归于9个属,内生真菌的多样性指数H=1.31、D=0.93,以毛壳菌属(Chaetomium)为优势菌群,占总菌株数的23.73%。由此可见,北京大花杓兰中内生真菌多样性高于吉林大花杓兰。The composition and diversity of endophytic fungal taxa in Cypripedium macranthum Sw was analyzed. The results showed that a total of 59 fungal strains were isolated from C. macranthum. These fungi were belonged to 18 taxa containing 7 classes, 9 orders, 11 families and 16 generas with analyzing ITS-rDNA sequence. Endophytic fungal groups in Beijing C. Macranthum included to 12 generas, with the diversity index H of 1.39 and D of 0.96. Chaetomium （15.25%） and Phoma （8.47%） were two major fungal groups. Endophytic fungal groups in Jilin C. Macranthum included 9 generas, with the diversity index H of 1.31 and D of 0.93.Chaetomium belonged to the core group（23.73%）. Diversity of endophytic fungi in Beijing C. Macranthum was higher than that of Jilin C. Macranthum.

关 键 词：大花杓兰(Cypripedium macranthum Sw.) 内生真菌 分离 鉴定 多样性 

分 类 号：Q939.5[生物学—微生物学]