组培和大田条件下人参、西洋参叶片解剖结构比较  

Comparative Study of Leaf Anatomical Structures of Panax ginseng C. A. Meyer and Panax quinquefolius L. Grown in vitro and Field

作　　者：尹红新[1] 雷秀娟[1] 宋娟[1] 王晗[1] 王英平[1] 姚春林[1] 

机构地区：[1]中国农业科学院特产研究所,长春130112

出　　处：《吉林农业大学学报》2015年第2期171-176,共6页Journal of Jilin Agricultural University

基　　金：吉林省青年科研基金项目(20140520165JH)

摘　　要：以人参、西洋参组培和大田苗叶片为试材，采用改良石蜡切片法和印迹法比较了组培和大田条件下人参、西洋参叶片解剖结构的变化。结果表明：两种生长环境下供试人参、西洋参叶片细胞层数均为5～6，组培条件下供试人参、西洋参叶片无栅栏组织，叶肉细胞大、胞间隙小、结构致密、细胞多为横向排列；而大田条件下供试人参、西洋参叶片在靠近上表皮处有1层栅栏组织，表皮和叶肉细胞小，胞间隙大、结构疏松，细胞多为纵向排列；组培和大田条件下，人参叶片气孔密度分别为86.50／mm2、22.30／mm2，西洋参叶片的气孔密度分别为74.80／mm2、33.30／mm2，均差异极显著。The modified paraffin section method and blotting method were used to study the anatomi-cal structure changes induced by growing conditions of Panax ginseng. C. A. Meyer and Panax quin-quefolius L. leaves in vitro and field. The results showed that in different growth environments, both Panax ginseng and Panax quinquefolius had 5 or 6 layers of mesophyll cells. Compared with the leaf grown in field, the epidermal cells and mesophyll cells of leaf grown in vitro were relatively large and their array was quite regular and close together, and there was no differentiation of palisade and spongy tissue. The average stomata density of Panax ginseng and Panax quinquefolius leaf grown in field was 22.30/mm2 and 33.30/mm2 and significantly lower than that of ginseng and Panax quin-quefolius grown in vitro （86.50/mm2 and 74.80/mm2）.

关 键 词：人参 西洋参 叶片 解剖结构 气孔 

分 类 号：S532.01[农业科学—作物学]