芪柴煎剂对变应性鼻炎大鼠血清IL-4,IFN-γ影响的实验研究  

Effect of Qi Chai decoction on serum IL-4, IFN-γ level in allergic rhinitis rats

作　　者：胥彪[1] 胡文健[2] 

机构地区：[1]达州市中西医结合医院耳鼻咽喉头颈外科,四川达州635000 [2]泸州医学院附属中医医院耳鼻咽喉科,四川泸州646000

出　　处：《泸州医学院学报》2015年第2期124-126,共3页Journal of Luzhou Medical College

摘　　要：目的:研究芪柴煎剂(自拟方)对变应性鼻炎大鼠模型血清IL-4、IFN-γ的影响,探讨其对变应性鼻炎大鼠的作用疗效。方法:应用卵清蛋白将实验大鼠制作成变应性鼻炎模型,随机分为正常对照组(A组)、芪柴煎剂低剂量组(B组)、高剂量组(C组)、地氯雷他定组(D组)、模型对照组(E组)各8只。连续14 d分别给予对应的药物,A组、E组以蒸馏水替代。用双抗体夹心ELISA法检测血清IL-4、IFN-γ的含量。结果:各实验组血清IL-4含量较模型对照组明显偏低,差异均有统计学意义(P<0.05);芪柴煎剂低剂量组IL-4含量高于芪柴煎剂高剂量组,差异有统计学意义(P<0.05)。各实验组血清IFN-γ含量高于模型对照组,差异均有统计学意义(P<0.05);芪柴煎剂低剂量组、地氯雷他定组IFN-γ含量低于高剂量组,差异有统计学意义(P<0.05)。结论:芪柴煎剂能够下调变应性鼻炎大鼠血清IL-4水平;上调IFN-γ水平。Objective： To study the influence of Qi Chai decoction on serum IL-4, IFN-γ level of allergic rhinitis model of rats so as to explore its role and efficacy in treating allergic rhinitis. Methods：Rats with allergic rhinitis were made by ovalbumin as sensitizing agent. 40 healthy adult SD rats were randomly divided into five groups（8 rats each）：normal control group low-dose and high dose Qi Chai decoction groups,loratadine group, and model control group. Corresponding mjodel control given for 14 consecutive days in each group. Then the serum IL-4 and IFN-γ contents were tested by ELISA. Results： Compared with model control group,the serum IL-4 levels of low dose and high dose Qi Chai decoction groups and desloratadine group were significantly lower, and there were significant differences （P〈0.05）, and IL-4 level of low dose Qi Chai decoction group was higher than that of high dose group （P〈 0.05）. Compared with model control group, the serum IFN-γ level of low dose and high dose Qi Chai decoction groups, and the desloratadine group were significantly higher （P〈 0.05）. Compared with high dose group, the serum IFN-γ level of low dose Qi Chai decoction group and loratadine group were significantly lower （P〈 0.05）. Conclusion： The Qi Chai decoction can down-regulate the serum IL-4 levels and up-regulate the serum IFN-γlevels of the rats with allergic rhinitis.

关 键 词：IL-4 IFN-Γ 变应性鼻炎大鼠模型 芪柴煎剂 

分 类 号：R246.81[医药卫生—针灸推拿学]