补肾法对肾虚大鼠空间学习记忆能力的影响  

Effect of Kidney-tonifying Therapy on Spatial Learning and Memory Abilities of Kidney Deficiency Rats

作　　者：吉云鹏[1] 徐志伟[1] 敖海清[1] 陈攀[2] 胡海燕[1] 吴皓萌[1] 张晨[1] 

机构地区：[1]广州中医药大学,广东广州510006 [2]广西中医药大学,广西南宁530001

出　　处：《广州中医药大学学报》2015年第3期464-468,577,共6页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金资助项目(编号:81173144);教育部2010年度新世纪优秀人才支持计划项目(编号:NCET-100088)

摘　　要：【目的】探讨补肾法对房室不节肾虚大鼠空间学习记忆能力及海马DG区细胞超微结构的影响。【方法】将连续配种6个月经鉴定生殖功能下降的SD大鼠随机分为肾虚模型组,六味地黄丸低、高剂量组(剂量分别为4.8、9.6 g·kg-1·d-1)和金匮肾气丸低、高剂量组(剂量分别为5.2、10.4 g·kg-1·d-1),并以2月龄大鼠作为正常对照组。连续给药30 d后,进行Morris水迷宫实验,采用透射电镜观察海马DG区细胞超微结构。【结果】Morris水迷宫定位航行实验结果显示:与正常对照组比较,肾虚模型组逃避潜伏期显著延长(P<0.01);与肾虚模型组比较,各给药组潜伏期显著缩短(P<0.05或P<0.01)。空间探索实验结果显示:与正常对照组比较,肾虚模型组原平台所在象限停留时间和平台周边区域一停留时间均显著减少(P<0.01);与肾虚模型比较,金匮肾气丸高剂量组2项停留时间均显著增加(P<0.05或P<0.01),六味地黄丸低剂量组平台周边区域一停留时间显著增加(P<0.05)。电镜观察结果显示:与肾虚模型组比较,六味地黄丸高剂量组和金匮肾气丸高剂量组可减轻大鼠海马DG区细胞超微结构病变。【结论】(1)长期房室不节可引起海马DG区细胞超微结构病变,导致空间学习记忆障碍。(2)补肾法可减轻房室不节对大鼠空间学习记忆能力的损伤及海马DG区细胞超微结构病变。(3)补肾方药在改善肾虚大鼠空间学习能力及细胞超微结构病变方面无显著性差异,金匮肾气丸改善房室不节肾虚大鼠的空间记忆能力的作用优于六味地黄丸。Objective To observe the effects of kidney-tonifying therapy on spatial learning, memory abilities and ultrastructure of hippocampal dentate gyrus （ DG ） cells of kidney deficiency rats induced by sexual intemperance. Methods SD rats with low reproductive capacity were caused by continuously mating for 6 months, and then were randomly divided into 5 groups, namely model group, low- and high-dose of Liuwei Dihuang pills （LDP） groups （in the dosage of 4.8, 9.6 g·kg-1·d-1）, low-and high-dose of Jingui Shenqi pills （JSP） group （in the dosage of 5.2, 10.4 g·kg-1·d-1）, 8 rats in each group. Except the model group, the other groups were respectively given with the corresponding medication. SD rats aged 2 months served as the normal control group. After treatment for 30 continuous days, the learning and memory abilities of rats were examined by Morris Water Maze. The cell ultra structure of hippocampal dentate gyrus was observed by transmission electron microscopy. Results The results of the place navigation test of Morris water maze showed that the escape latency of rats was prolonged obviously in the model group compared with that of the normal control group （P〈0.01）, and the escape latency of rats in the medication groups was shorter than the kidney deficiency model group （P〈0.05 or P〈0.01）. The results of spatial probe test showed that the residence time in the former hidden platform quadrant and its surrounding area was significantly shortened in the model group compared with that of the normal control group （ P〈0.01） , but was prolonged after medication of high-dose JSP （ P〈0.05 or P〈0.01 compared with that of the model group）. Under the electron microscope, the cell ultra structure lesions of hippocampal dentate gyrus were much relieved in the high-dose LDP group, and high-dose JSP group as compared with those of the model group. Conclusion （ 1） Long-term sexual intemperance can impair spatial learning and memory abilities and causes the cell ultra structure

关 键 词：补肾法 肾虚/中药疗法 房室不节 MORRIS水迷宫 学习记忆能力 海马/超微结构 疾病模型  动物 大鼠 

分 类 号：R285.5[医药卫生—中药学]