龟板提取物诱导神经干细胞向神经元分化过程中相关microRNA表达变化  

Changes of micro RNA Expression During Neuronal Differentiation of Neural Stem Cells Induced by Plastrum Testudinis Extract

作　　者：李彩霞[1] 周健洪[1] 陈东风[1] 黎晖[1] 张娴[2] 黄羽[2] 

机构地区：[1]广州中医药大学基础医学院,广东广州510006 [2]广州中医药大学附属广东省中医院中心实验室,广东广州510006

出　　处：《广州中医药大学学报》2015年第3期481-484,573-575,共7页Journal of Guangzhou University of Traditional Chinese Medicine

基　　金：国家自然科学基金项目(编号:81303116);广东省自然科学基金项目(编号:S2011040004778);广州中医药大学创新基金项目(编号:2005c002)

摘　　要：【目的】观察龟板提取物诱导神经干细胞(neural stem cells,NSCs)向神经元细胞定向分化过程中相关micro RNA表达的变化。【方法】分离培养孕14 d SD胎鼠原代神经干细胞,经免疫荧光染色鉴定神经干细胞的特异抗原及其多向分化能力。神经干细胞随机设空白对照组、龟板提取物低浓度组(3μg/m L)、龟板提取物高浓度组(30μg/m L)。诱导分化7 d,提取各组细胞总RNA,采用实时荧光定量PCR法检测不同组别mi R-124和mi R-9的变化。【结果】与空白对照组比较,龟板提取物低浓度组mi R-124和mi R-9表达均无显著变化,龟板提取物高浓度组均显著升高(P<0.05)。【结论】龟板提取物可能通过调控mi R-124、mi R-9表达在NSCs向神经元细胞定向分化过程中起重要作用。Objective To explore the changes of microRNA expression during the neuronal differentiation of neural stem cells （NSCs） induced by Plastrum testudinis extract （PTE） . Methods NSCs were isolated from the hippocampus of 14-day SD rat fetus and were obtained after primary culture. The abilities of NSCs differenting into neurons were identified by immunofluorescent staining. NSCs were randomly divided into three groups, blank control group, and low- and high-dose PTE groups （ 3, 30 μg/mL PTE） . After the cells were incubated with PTE for 7 days, total RNA was isolated and then the expression of miR-124 and miR-9 was observed by real-time fluorescence quantitative polymerase chain reaction （ qRT-PCR） . Results Compared with the blank control group, the expression of miR-124 and miR-9 remained unchanged in low-dose PTE group, but was significantly increased in high-dose PTE group （ P〈0.05）. Conclusion PTE promotes NSCs differentiating into neurons, which might be associated with the up-regulation of miR-124 and miR-9.

关 键 词：龟板提取物/药理学 神经干细胞/病理学 基因表达调控 细胞培养 大鼠 

分 类 号：R285.5[医药卫生—中药学]