高糖环境肾小管上皮细胞Smad1的表达  

Expression of Smad1 in renal tubular epithelial cells in high glucose condition

作　　者：陈梅莲[1] 付文金[1] 谢岭平[1] 王杜娟[1] 李柳燕[1] 邓任堂[1] 

机构地区：[1]广东医学院附属厚街医院检验科,广东东莞523945

出　　处：《中国热带医学》2015年第1期8-10,共3页China Tropical Medicine

基　　金：东莞市重点课题项目(No.2012105102013)

摘　　要：目的模拟糖尿病条件,观察人肾小管上皮细胞(HK-2)生长情况和Smad1表达的变化,探讨高糖对肾小管上皮细胞的损伤机制。方法用DMEM/F12细胞液培养HK-2细胞,分为对照组,中糖组(12.5mmol/L葡萄糖),高糖组(25mmol/L葡萄糖),分别培养24h、48h、72h后倒置显微镜下观察HK-2的形态和数量变化,采用蛋白印迹技术检测Smad1的蛋白表达。结果高糖组刺激72h后,与对照组相比HK-2细胞数为(105.333±6.429)×105/m L,而对照组HK-2细胞数为(50.333±2.51)×105/m L,差异有统计学意义(P<0.01);高糖组HK-2细胞形态改变,Smadl蛋白表达明显增加。结论高糖能够诱导肾小管上皮细胞数量及形态发生改变,并且这一作用可能与Samd1表达增加有关。Objective To observe the growth and expression of Smad1 in human renal tubular epithelial（HK-2）cellsunder high glucose condition, and to evaluate the possible injured mechanisms of high glucose in HK-2. Methods HK-2cells were cultured in DMEM/F12 medium, and divided into control group,medial glucose group（12.5 mmol/L D- glucose） andhigh glucose group（25 mmol/L D- glucose）, and then each was cultured for 24 h, 48 h and 72 h respectively. Cell numbers andmorphological changes were traced with inverted microscope. Western blot was applied to detect the protein expression levelsof Smad1. Results Compared to the cells in control group the shape of HK- 2 cells in high glucose group 72 hours afterinduced by high glucose changed and the numbers of HK-2 cells increased markedly（10^5.333±6.429）×10^5/m L, exhibitingstatistically significant difference（P〈0.01）, also the protein expression of Smad1 in high glacose group was up- regulatedsignificantly（0.430±0.05）compared with that of the control group（0.123±0.008）,showing significant difference（P〈0.01）.Conclusion High glucose can induce morphological and number changes of HK- 2 cells and this function might beassociated with the increased expression of Smad1.

关 键 词：高糖 肾小管上皮细胞 SMAD1 糖尿病肾病 

分 类 号：R587[医药卫生—内分泌]