手足口病患儿淋巴细胞亚群及Th1/Th2型细胞因子表达  

Expression and significance of the lymphocyte subsets and Th1/Th2 type cytokines in children with HFMD

作　　者：杜潘艳[1] 赵丽梅[1] 乔国昱 王晓波[1] 

机构地区：[1]唐山市妇幼保健院检验科,河北唐山063000 [2]唐山市协和医院检验科,河北唐山063000

出　　处：《中国热带医学》2015年第1期22-24,共3页China Tropical Medicine

摘　　要：目的检测手足口病患儿外周血中淋巴细胞亚群及Th1/Th2型细胞因子水平,探讨其临床意义。方法应用流式细胞仪检测淋巴细胞亚群CD3+、CD4+、CD8+、CD19+和CD16+CD56+百分比;采用酶联免疫吸附试验(ELISA)检测细胞因子IFN-γ、IL-2、IL-4和IL-10水平。结果病例组患儿CD3+、CD4+、CD8+和CD16+CD56+淋巴细胞亚群百分比均低于对照组(P<0.05);危重型病例组淋巴细胞亚群CD3+、CD4+、CD8+和CD16+CD56+百分比均低于重型病例组,且两组均低于普通病例组(P<0.05);危重型病例组CD19+淋巴细胞亚群百分比高于重型病例组,且两组均高于普通病例组和对照组(P<0.05),普通病例组和对照组间无统计学意义(P>0.05)。病例各组患儿细胞因子IFN-γ、IL-2、IL-4和IL-10水平均高于对照组(P<0.01);危重型病例组IFN-γ、IL-2、IL-4和IL-10水平均高于重型组和普通病例组,且重型病例组高于普通组(P<0.01)。淋巴细胞亚群CD3+、CD4+、CD8+和CD16+CD56+百分比与病情呈负相关(r=-0.942、r=-0.953、r=-0.874、r=-0.953,P<0.01);CD19+、IFN-γ、IL-2、IL-4和IL-10水平与病情呈正相关(r=0.659、r=0.937、r=0.946、r=0.891、r=0.879,P<0.01)。结论 HFMD患儿细胞免疫和体液免疫功能异常,免疫功能降低是HFMD发病的主要原因之一,而且也是引起HFMD不同临床经过的关键因素。Objective To detect the lymphocyte subsets and Th1/Th2 type cytokines levels in the peripheral blood inthe children with hand- foot- mouth disease（HFMD） and explore its clinical significance. Methods The percentage oflymphocyte subsets CD3^＋, CD4^＋,CD8^＋, CD19^＋and CD16^＋CD56^＋were detected by flow cytometry; the levels of cytokines IFN-γ, IL- 2, IL- 4 and IL- 10 were detected by ELISA. Results The percentage of CD3^＋, CD4^＋, CD8^＋and CD16^＋CD56^＋lymphocyte subsets in the case group were lower than that in the control group（P〈0.05）, respectively; the percentage of CD3^＋,CD4^＋, CD8^＋and CD16^＋CD56^＋lymphocyte subsets in the critical group were lower than those in the severe group, and those intwo groups were lower than that in the mild group（P〈0.05）, respectively. But the percentage of CD19^＋lymphocyte subsets inthe critical group were higher than in the severe group, and those in two groups were higher than that in the mild and controlgroup（P〈0.05）, there was no difference between the mild group and control group（P〈0.05）. The cytokines levels of IFN-γ, IL-2, IL-4 and IL-10 in the case group were higher than those in the control group（P〈0.01）, respectively. The levels of the IFN-γ, IL-2, IL-4 and IL-10 in the critical group were higher than that in the severe and mild group, and the cytokines level in thesevere group were higher than that in the mild group（P〈0.01）, respectively. The percentage of lymphocyte subsets CD3^＋, CD4^＋,CD8^＋and CD16^＋CD56^＋were negatively related to the state of HFMD（r =-0.942, r =-0.953, r =-0.874, r =-0.953, P〈0.01）; thelevel of CD19^＋, IFN- γ, IL-2, IL-4 and IL-10 were positively correlated to severity of HFMD（r =0.829, r = 0.937, r =0.946, r =0.891, r =0.879,P〈0.01）. Conclusion There were function abnormalities of cellular and humoral immune in the childrenwith HFMD, reduction of immune function is one of the main reasons for the onset of HFMD, but also it is a key factors for thediff

关 键 词：手足口病 免疫功能 淋巴细胞亚群 TH1/TH2型细胞因子 

分 类 号：R512.5[医药卫生—内科学]