疟原虫感染组织切片吉姆萨染色法的建立及优化  

Establishment and optimization of Giemsa staining of tissue infected by Plasmodium berghei

作　　者：黄宪希[1] 周利民[1] 易国辉[1] 薛伟玲[1] 郭虹[1] 

机构地区：[1]海南医学院科学实验中心,海南海口571199

出　　处：《中国热带医学》2015年第1期91-93,共3页China Tropical Medicine

基　　金：国家自然科学基金(No.81060140);海南医学院科研启动基金(No.2011011)

摘　　要：目的探讨使用吉姆萨(Giemsa)染色法对伯氏疟原虫(Plasmodium berghei,P.b.)ANKA株感染小鼠肝、脾组织标本进行染色的可行性及效果。方法小鼠肝、脾组织用10%福尔马林固定后石蜡包埋,4μm厚切片,60℃烤箱30min烘烤,二甲苯脱蜡,常规乙醇水化至水,用2、3、4倍稀释的吉姆萨工作液分别染色3min、5min、10min;同时做常规苏木精-伊红(Haematoxylin-eosin,HE)染色。结果 3倍稀释Giemsa工作液染色5min的染色效果最佳。镜下观察,疟原虫染色鲜明、虫体胞浆蓝色,胞核粉红、疟色素染为棕褐色,清晰易辨,明显优于HE染色法。结论使用Giemsa染色法对P.b.感染小鼠肝、脾组织标本的染色简单易行,需时短,效果良好,在临床及科研领域中具有应用价值。Objective To explore the feasibility and effectiveness of Giemsa staining of mouse liver and spleen tissuesinfected by Plasmodium berghei（P.b.） ANKA strain. Methods Mouse liver and spleen tissues were fixed in 10% formalin,embedded in paraffin, and then cut into 4μm- thick slices. The slices were placed in oven at 60℃ for 30 minutes, thendeparaffinized in xylene, rehydrated through an ethanol series to water. The rehydrated slices were stained with 2- fold, 3-fold and 4-fold diluted Giemsa working solution for 3, 5 and 10 minutes, respectively. Routine HE staining was done at thesame time. Results Samples stained with 3- fold diluted Giemsa solution for 5 minutes showed satisfied results. Thecytoplasm of P.b. on the slices was stained blue, the nucleus was dyed pink and the malaria pigment was colored brown, clearlegibility. The Giemsa staining approach achieved significantly better results than that of HE. Conclusions The Giemsastaining is a feasible and efficient method for staining mouse liver and spleen tissues infected with P.b. It is a useful techniquefor both clinical and scientific researches.

关 键 词：伯氏疟原虫 吉姆萨染色法 HE染色法 

分 类 号：R531.3[医药卫生—内科学]