水菖蒲水提物的抗炎与促愈活性研究  

Anti-inflammatory and Wound-healing Activity of Aqueous Extracts from Acorus Calamus L

作　　者：王常丽[1] 吴琼[1] 陈宇峰[1] 张威[1] 赵庆春[1] 

机构地区：[1]沈阳军区总医院药剂科,沈阳110016

出　　处：《中国药师》2015年第5期730-733,共4页China Pharmacist

摘　　要：目的:研究新鲜水菖蒲根茎水提物的创伤促愈和抗炎活性。方法:应用切创模型考察水菖蒲创伤愈合作用,以图像分析技术和免疫组化进行分析,应用脂多糖(LPS)制备RAW264.7细胞系炎症模型,通过RT-PCR技术检测炎症因子水平,从而考察其抗炎活性。结果:于动物创伤模型日3次给药,3 d起给药组较对照组有创面面积减少,13 d后仅为对照组面积的15%,显著提高创伤治愈率,同时由脂多糖诱导的RAW 264.7炎症可被水菖蒲水提物有效抑制,给药组与对照组相比炎症因子TNF-α,IL-1β,IL-6的m RNA表达均下调,并呈现一定的剂量依赖性。结论:自新鲜水菖蒲根部的水提物在小鼠切创模型上表现出明显的促愈作用,同时在LPS诱导的炎症模型上体现了明显的抗炎作用。Objective：To explore the wound-healing effect and the anti-inflammatory activity of the aqueous extracts from the fresh roots and rhizomes of A. calamus. Methods： The image analysis techniques and the histological analysis were used to determine the wound-healing effect in the excised wound test, and the real-time RT-PCR techniques was used to evaluate the anti-inflammatory activi-ty in the lipopolysaccharide-induced RAW 264. 7 cells test. Results：The aqueous extracts were given 3 times a day since the model was established. The skin wound area was reduced significantly in the aqueous extracts group when compared with that in the control group since the 3rd day, the wound area in the aqueous extracts group was only 15% of that in the control group on the 13th day, and the wound-healing rate was enhanced significantly by the extracts. Moreover, the mRNA expressions of the inflammatory mediators such as TNF-α, IL-1β and IL-6 in the inflammatory RAW 264. 7 cells induced by lipopolysaccharide were inhibited effectively by the ex-tracts in a dose-dependant manner. Conclusion：The results indicate that the aqueous extracts from the fresh roots and rhizomes of A. calamus have significant wound-healing activity in animal excised wound model and anti-inflammatory activity in vitro.

关 键 词：水菖蒲水提物 促愈 抗炎 

分 类 号：R285.5[医药卫生—中药学]