化生平胶囊的质量标准研究  

Study on Quality Standard for Huashengping Capsules

作　　者：车晓侠[1] 席枝侠[1] 王娜[1] 赵宇[1] 蔡艳[1] 常权 

机构地区：[1]西安交通大学第二附属医院,西安710004 [2]西安医学院

出　　处：《中国药师》2015年第5期733-736,共4页China Pharmacist

基　　金：陕西省中医药管理局基金资助(编号:zy28)

摘　　要：目的：建立化生平胶囊的质量标准。方法：采用TLC法对黄芪、白花蛇舌草、丹参进行鉴别；用HPLC法测定黄芪甲苷的含量,色谱柱为Kromasil C18（250 mm ×4.6 mm,5μm）,流动相为乙腈-水（32∶68）,流速：1.0 ml·min-1,检测波长：203 nm柱温：25℃,进样量：20μl。结果：TLC色谱斑点清晰；黄芪甲苷在进样量为2.000~10.000μg 范围内线性关系良好（ r=0.9996）,平均回收率为100.8%,RSD为1.9%（n=6）。结论：该方法简便、准确、可靠,可用于化生平胶囊的质量控制。Objective：To establish the quality standard for Huashengping capsules. Methods： Milkvetch Root, Hedyotis diffusa Willd and Salvia miltiorrhiza were identified by TLC. The content of astragaloside A was detected by HPLC. The column was Kormasil C18（250 mm ×4.6 mm, 5 μm） and the flow rate was 1.0 ml·min-1. The mobile phases was a mixture of acetonitrile-water （32∶68 ） . The detection wavelength was 203 nm. The column temperature was 25℃ and the sample size was 20μl. Results：The TLC chro-matography spots were clear. Astragaloside A was linear within the range of 2. 000-10. 000 μg（r=0. 999 6） and the average recovery was 100. 8%（RSD=1. 9%,n=6）. Conclusion：The method is simple, accurate and reliable, which can be used in the quality con-trol of Huashengping capsules.

关 键 词：化生平胶囊 薄层色谱法 高效液相色谱法 

分 类 号：TQ460.72[医药卫生—药物分析学]