不同产地香附中总黄酮含量比较研究  

Content Comparison of Total Flavonoids in Cyperus Rotundus from Different Habitats

作　　者：曹玫[1] 欧阳露[1,2] 

机构地区：[1]湖北医药学院附属人民医院,湖北十堰442000 [2]湖北医药学院药学院

出　　处：《中国药师》2015年第5期773-774,共2页China Pharmacist

基　　金：湖北省教育厅项目(编号:B20082412);湖北省2011协同创新中心基金(编号:4);十堰市科学技术研究与开发项目(编号:ZD2011023)

摘　　要：目的：建立香附总黄酮含量检测方法,并考察不同产地香附中总黄酮的含量。方法：以芦丁作为标准品,紫外分光光度法检测不同产地香附中总黄酮含量。结果：芦丁对照品浓度在0.0643~0.6426 mg·ml-1范围内与吸光度呈良好的线性关系（r=0.9991）,加样回收率为99.74%,RSD为2.16%（n=6）。不同产地香附总黄酮含量依次是河南〉四川〉广东〉安徽〉海南〉山东。结论：该方法简便、准确,可用于香附的质量控制。香附总黄酮含量以河南产地最高。Objective：To establish the determination method for total flavonoids in Cyperus rotundus and investigate the total fla-vonoids content in Cyperus rotundus from different habitats. Methods：Total flavonoids in Cyperus rotundus from different habitats were detected by ultraviolet spectrophotometry with rutin as the standard substance. Results：The absorbance of rutin had a good linear cor-relation with the concentration within the range of 0.064 3-0.642 6 mg·ml-1（r =0.999 1）. The average recovery was 99.74%（RSD=2. 16%,n=6）. The order of total flavonoids content in Cyperus rotundus from different habitats was Henan province 〉Sichuan province〉 Guangdong province 〉Anhui province 〉 Halnan province 〉Shandong province. Conclusion：The method is easy and ac-curate, which can be applied in the quality control of Cyperus rotundus. The total flavonoids content in Cyperus rotundus from Henan province is the highest.

关 键 词：香附 总黄酮 紫外分光光度法 

分 类 号：R927.2[医药卫生—药学]