基于微电子神经桥重建大鼠皮质脊髓束功能的体视学定量研究  

Quantitative stereological measurement of the rat corticospinal tract based on functional reconstruction using microelectronic neural bridge method

作　　者：刘晓东[1] 李耀富[2] 吴辉群[3] 季达峰[1] 韩笑[1] 王昊亮[4] 许媛媛[4] 谢天[4] 孟祥宇[4] 毛玉娣 赵宁[4] 顾娟娟[4] 吕广明[1] 

机构地区：[1]南通大学医学院人体解剖学系,南通226001 [2]南通大学教务处,南通226001 [3]南通大学医学院医学信息学系,南通226001 [4]南通大学医学院,南通226001

出　　处：《南通大学学报（医学版）》2015年第2期98-101,共4页Journal of Nantong University(Medical sciences)

基　　金：国家自然科学基金重点项目(90707005);江苏省高等学校大学生实践创新训练计划立项项目(2012JSSPITP1550);南通大学研究生培养创新工程项目(YKC13061)

摘　　要：目的：获取大鼠皮质脊髓束的立体定位解剖学数据。方法：取成年SD大鼠脊髓T6~10节段连续冰冻切片，通过蛋白激酶Cγ（protein kinase Cγ, PKCγ）免疫组织化学法染色显示皮质脊髓束，运用体视学方法定量测量，获取各节段皮质脊髓束距脊髓横切面正中线的最大距离、距脊髓背面的最小距离和最大距离以及皮质脊髓束的截面积等皮质脊髓束定位的相关数据。结果：在PKCγ免疫组织化学染色中，皮质脊髓束呈棕黄色，定位于大鼠脊髓后索最深部，边界清晰，易于定位。体视学方法测量获得T6~10节段脊髓中央管到脊髓背面距离（h），两侧皮质脊髓束的截面积（s, s’），双侧皮质脊髓束距脊髓中线的距离（a, a’）、皮质脊髓束距脊髓背面的最小距离（b, b’）、皮质脊髓束距脊髓背面的最大距离（c, c’），左侧与右侧相比差异无统计学意义（P＞0.05）。结论：用PKCγ免疫组织化学染色法能准确定位皮质脊髓束，体视学定量测量可获取大鼠皮质脊髓束的立体定位解剖学数据，为脊髓损伤后植入微电子神经桥治疗提供应用解剖学基础。Objective：To obtain the stereotactic anatomic data of the rat corticospinal tract （CST）. Methods：Adult SD rats were selected, and the continuous cyrosections from thoracic segment 6（T6） to 10（T10） of the spinal cord were conducted. After protein kinase Cγ（ PKCγ） immunohistochemistry （ IHC ） staining , the stereotactic data of CST were quantitatively measured with stereological methods. On each spinal cord segment transversal section, the largest distance between CST to the midline, the shortest as well as largest distance between CST to dorsal surface of spinal cord, and the sectional area of CST were recorded. Results： After PKCγ IHC staining, the CST was shown in brown, and was confined to the deepest part of the posterior funiculus of spinal cord with clear boundary and was easy to be located. Quantitative stereological results showed that there was no statistical significance among the distances between the central canal to dorsal surface of spinal cord（h）, the sectional area of bilateral CSTs（s, s’）, the distances between bilateral CSTs to midline （a, a’）, and the largest distance as well as the shortest distance between CST and spinal dorsal surface （b, b’;c, c’） from T6~10 bilaterally （P〉0.05）. Conclusion：PKCγimmunohistochemical staining method can locate the corticospinal tract accurately, and quantitative stereological measurement could obtain the stereotactic data of corticospinal tract, and provide applied anatomical basis for therapy research on spinal cord injury using implantation of microelectronic neural bridge.

关 键 词：皮质脊髓束 蛋白激酶CΓ 免疫组织化学 体视学定量测量 微电子神经桥 大鼠 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学]