Ⅱ型胶原蛋白酶诱导SD大鼠膝骨关节炎模型的建立  

Establishment of SD rat model on knee osteoarthritis induced by type Ⅱ collagen enzyme

作　　者：黄永明[1] 潘建科[2] 郭达[1] 刘军[1] 杨济源[2] 洪坤豪 杨伟毅[1] 许树柴[1] 

机构地区：[1]广东省中医院骨科,广州510120 [2]广州中医药大学第二临床医学院,广州510405

出　　处：《广东医学》2015年第8期1145-1148,共4页Guangdong Medical Journal

基　　金：国家自然科学基金资助项目(编号:81273781;81473698);教育部高等学校博士学科点专项科研基金资助课题(博导类)(编号:20124425110004);2011年广东省第一批科学事业费计划项目(编号:2011B031700027);广东省财政厅"中老年退行性膝骨关节炎中医药防治管理体系"项目(编号:[2014]157号);2013年度广东省中医院中医药科学技术研究专项(第二批)立项课题(编号:YK2013B2N19)

摘　　要：目的建立Ⅱ型胶原蛋白酶诱导的SD大鼠膝骨关节炎模型。方法将SD大鼠20只随机分为A组和B组,每组10只。分别于第1、4天给予A组大鼠膝关节腔内注射Ⅱ型胶原蛋白酶,予B组大鼠膝关节腔内注射生理盐水进行造模。观察两组大鼠第1、3和7天体重的变化,以及第1、4、7天膝关节直径的变化,测定第7天大鼠处死后脾脏、胸腺重量和脏器系数,HE染色观察光镜下膝关节组织的一般形态。结果造模后,A组的体重呈下降、减少趋势,B组的体重呈上升、增长趋势。造模后第3、7天,A、B组间体重比较差异有统计学意义。A组造模后的膝关节直径明显大于造模前,前后比较差异有统计学意义(P<0.01)。B组造模后的膝关节直径与造模前差异无统计学意义(P>0.05)。A组造模后的膝关节直径大于B组,差异有统计学意义(P<0.01)。关节组织HE染色光镜下观察显示,A组的关节呈早期骨关节炎的表现,而B组的关节组织无骨关节炎的表现。结论Ⅱ型胶原蛋白酶可成功建立SD大鼠早期膝骨关节炎模型。体重、膝关节直径、脾脏和胸腺重量、脏器系数及膝关节组织病理这几个指标有助于此模型生理病理特点的观察。Objective To establish the knee osteoarthritis（OA） model using type Ⅱ collagen enzyme in SD rats. Methods Twenty SD rats were randomly divided into Group A（ model group） and Group B（ normal saline control group）,in which type Ⅱ collagen enzyme and saline were given by knee injection,respectively,on the 1^st and 4^th day.Body weight on the 1^st,3^rd and 7^th day,knee joint diameter on the 1^st and 4^th day were recorded. The subjects were sacrificed on the 7^th day for assessment of spleen and pancreas weights,and viscera coefficient,while the the general morphology of the knee joint tissues with HE staining was also observed. Results After modeling,body weight reduced in Group A,while it increased in Group B. The knee joint diameter was significantly larger after modeling than before modeling in Group A（ P 〈0. 01）,while there was no significant difference revealed in Group B（ P〉 0. 05）. Knee joint diameter in Group A was significantly larger than that in Group B after the modeling（ P〈0. 01）. Early OA was observed in Group A,while no evidence was revealed in Group B. Conclusion By using collagen type Ⅱ enzymes,early knee OA model can be successfully established in SD rats. Body weight,knee joint diameter,spleen and pancreas weights,organ coefficient and knee joint pathology could be applied for study of the pathophysiological features of this OA model.

关 键 词：胶原蛋白酶 膝骨关节炎 关节软骨 模型 

分 类 号：R593.22[医药卫生—内科学]