丹参经肝动脉灌注对大鼠移植性肝癌的治疗及其肝功能保护作用研究  

Efficacy of salvia miltiorrhiza on transplanted hepatoma in rats via hepatic artery perfusion and its protection of liver function

作　　者：魏明琴 蒋建霞[2] 

机构地区：[1]湖北医药学院附属襄阳医院肿瘤科,湖北襄阳441000 [2]南京医科大学,江苏南京210000

出　　处：《中国生化药物杂志》2015年第2期42-44,共3页Chinese Journal of Biochemical Pharmaceutics

基　　金：国家自然科学基金(81101800)

摘　　要：目的观察丹参经肝动脉灌注对大鼠移植性肝癌的治疗作用,并研究丹参在抗肿瘤的同时对移植性肝癌大鼠肝功能的保护作用。方法选用Sprague-Dawley雄性大鼠150只,并成功建立Walker-256移植性肝癌模型120只。所有造模大鼠随机分为对照组、丝裂霉素组和丹参组,每组40只。正常饲养10 d后,取各组大鼠血液检测血清ALT、AST和ALP水平,剖腹测量计算移植性瘤体体积。同时行经胃十二指肠动脉至肝脏固有动脉插管灌注治疗:对照组大鼠灌注2 m L的注射用生理盐水,丝裂霉素组大鼠灌注丝裂霉素(2.5 mg/kg),丹参组灌注丹参(1.6 g/kg)。灌注1周后,取血液检测ALT,AST和ALP水平,测量计算瘤体体积,并计算肿瘤增长率和抑制率。同时处死所有大鼠,取出瘤体,显微镜下观察肿瘤坏死范围并判断坏死程度。结果灌注治疗后,对照组、丝裂霉素组和丹参组大鼠分别剩余37只、37只和38只。丝裂霉素组和丹参组肿瘤体积及增长率均明显小于对照组(P<0.05),丝裂霉素组和丹参组肿瘤体积及增长率无明显差异。丝裂霉素组和丹参组对移植性肝癌肿瘤的抑制率分别为22.32%和26.38%,差异无统计学意义。灌注后,丹参组和丝裂霉素组大鼠中度坏死明显高于对照组,丝裂霉素组和丹参组轻、中、重肿瘤坏死分布无显著性差异。灌注后,丹参组和丝裂霉素组大鼠血清ALT,AST和ALP的水平均明显低于对照组,差异有统计学意义(P<0.01,P<0.05);丹参组上述指标显著低于丝裂霉素组,差异具有统计学意义(P<0.05)。结论丹参经肝动脉灌注对大鼠移植性肝癌有良好的抑制作用,其抑瘤活性与常用抗肿瘤药物丝裂霉素的活性相当。在抑制肿瘤的同时对移植性肝癌大鼠的肝功能也具有较好的保护作用。Objective To observe the therapeutic effect of salvia miltiorrhiza on transplanted hepatoma in rats via hepatic artery perfusion therapy and research the liver protection of salvia miltiorrhiza. Methods 150 Sprague-Dawley rats were selected to build Walker-256 transplanted hepatoma model with successful modeling of 120 rats. All rats were randomly divided into control group,mitomycin group,salvia miltiorrhiza group,and 40 rats in each group. After 10 days normal feeding,bloods of all rats were collected to detect levels of ALT,AST and ALP. Tumor volumes were measured at the same time. Hepatic arterial infusion therapy： 2 m L saline was injected into control group,mitomycin（ 2. 5 mg / kg） was injected into mitomycin group and salvia miltiorrhiza（ 1. 6 g / kg） was injected into salvia miltiorrhiza group. After a week,bloods were collected to detect levels of ALT,AST,ALP and tumor volumes were measured. The tumor growth rate and inhibition rate were calculated. At the same time,all rats were killed and tumor were removed. The scope of tumor necrosis and degree of tumor necrosis was observed and compared. Results There were 37 rats left in control group,37 rats left in mitomycin group and 38 rats left in salvia miltiorrhiza group after perfusion. The tumor volume and growth rate of mitomycin group and salvia miltiorrhiza group were significantly smaller than those of control group（ P 〈0. 05）,that had no significant difference between mitomycin group and salvia miltiorrhiza group. The tumor inhibition rate of mitomycin group and salvia miltiorrhiza group were 22. 32% and 26. 38%,and there was no statistical difference. After perfusion,the moderate degrees of tumor necrosis in mitomycin group and salvia miltiorrhiza group were higher than that in control group,and there were no significant difference in mitomycin group and salvia miltiorrhiza group in mild,moderate,and severe degrees of tumor necrosis. The levels of ALT,AST and ALP of mitomycin group and salvia miltiorrhiza group were lower than those o

关 键 词：丹参 肝动脉灌注 移植性肝癌 肝功能保护 

分 类 号：R969[医药卫生—药理学]