柴胡与氯苯那敏在大鼠体内药物间的相互作用研究  

Study of drug interaction between Bupleurum smithii extracts and chlorpheniramine in rats

作　　者：高柳柳 张晶[1] 徐外兰[1] 李玉良[1] 郭延垒[1] 于超[1] 

机构地区：[1]重庆医科大学生命科学研究院,重庆400016

出　　处：《中国生化药物杂志》2015年第2期165-167,170,共4页Chinese Journal of Biochemical Pharmaceutics

基　　金：国家自然科学基金(81370403)

摘　　要：目的探讨柴胡提取物与氯苯那敏在大鼠体内药物间的相互作用。方法将20只SD大鼠随机分为2组,实验组10只给予20 mg/kg的柴胡提取物2周,对照组10只平行给予生理盐水,第15天分别给予氯苯那敏,于(0、0.1、0.5、1、1.5、2、3、4、6、8、10、12、24 h)点采血200μL,采用LC-MS分析方法测定氯苯那敏的血药浓度,以Graphpad prism 5.01软件计算2组的药代动力学参数。结果实验组的AUC(0-∞)[1.40±0.27(μg·h)/m L]明显高于对照组[0.87±0.25(μg·h)/m L];而代谢清除率CLint[14.25±2.84(L·kg)/h]明显低于对照组[22.97±2.03(L·kg)/h](P<0.05)。结论柴胡提取物能够降低大鼠体内氯苯那敏的代谢清除率,临床上用药时应尽量避免2种药物的联合使用。Objective To investigate the drug-drug interaction between Bupleurum smithii extract and chlorpheniramine in rats. Methods 20 SD rats were randomized into two groups. 10 SD rats in experimental group were orally administered with Bupleurum smithii extract（ 20 mg / kg） once a day for two weeks and the other 10 rats in control group were orally administered with normal saline. On the 15 th day,20 SD rats were orally administered with chlorpheniramine. 200 μL plasma samples were collected at（ 0,0. 1,0. 5,1,1. 5,2,3,4,6,8,10,12,24 h） different time points. Plasma concentrations of chlorpheniramine were detected by LC-MS and pharmacokinetic parameters of 2 groups were calculated using Graphpad prism 5. 01.Results The AUC（ 0- ∞）of experimental group[1. 40 ± 0. 27（ μg·h） / m L]was higher than that of control group[0. 87 ± 0. 25（ μg·h） / m L],while the CLintof experimental group[14. 25 ± 2. 84（ L · kg） / h]was obviously lower than that of control group[22. 97 ± 2. 03（ L · kg） / h ]（ P〈0. 05）.Conclusion These results demonstrates that Bupleurum smithii extract can decrease the CLintof chlorpheniramine,clinical medication should try to avoid combination of 2 drugs.

关 键 词：柴胡提取物 氯苯那敏 CYP2D6 相互作用 

分 类 号：R969[医药卫生—药理学]