全细胞催化法生产N-乙酰神经氨酸的研究进展  

Advances in Production of N-acetyl-D-neuraminic Acid by Whole-cell Biocatalysis

作　　者：张丛丛[1] 陈彩霞[1] 陈笑[1] 温雅[1] 晏礼明[1] 陶勇[1] 

机构地区：[1]中国科学院微生物研究所,北京100101

出　　处：《生物技术通报》2015年第4期175-183,共9页Biotechnology Bulletin

摘　　要：N-乙酰神经氨酸(N-acetyl-D-neuraminic acid,Neu5Ac)及其衍生物不仅在人体内发挥重要的生理生化功能,且已经应用到流感的预防和治疗。但已有的N-乙酰神经氨酸生产方法产量低、成本高,限制了其大规模生产和广泛应用。随着分子生物学技术及糖组学研究的发展与成熟,一种新型的生物技术即全细胞催化法生产N-乙酰神经氨酸正成为研究的热点。旨在介绍全细胞催化技术合成N-乙酰神经氨酸的研究进展。N-acetyl-D-neuraminic acid and its derivatives have versatile biological functions and considerable contribution in the therapeutics field. But the production and wide applications of Neu5Ac are limited by the low yield and high cost. With the development of Molecular Biology and the research of Glycomics, the production Neu5Ac by whole-cell biocatalysis, a novel type of biocatalysis, has attracted researchers’attention. In this paper, we report that the advances in production of Neu5Ac by whole-cell biocatalysis, and also propose some guidelines for future studies.

关 键 词：N-乙酰神经氨酸 唾液酸 全细胞催化法 生物转化 

分 类 号：TQ460.6[化学工程—制药化工]