砷对人正常膀胱上皮细胞信号传导与转录激活因子3mRNA表达的影响  

Effects of arsenic on STAT3 m RNA expression in SV-HU-1 cells

作　　者：李梦丹[1] 刘婕瑜 王晨[1] 刘盛男[1] 王菲[1] 席淑华[1] 

机构地区：[1]中国医科大学公共卫生学院,辽宁沈阳110013

出　　处：《环境与健康杂志》2015年第2期99-101,共3页Journal of Environment and Health

基　　金：国家自然科学基金(81373023)

摘　　要：目的研究亚砷酸钠对人正常膀胱上皮(SV-HUC-1)细胞中信号传导与转录激活因子3(STAT3)m RNA表达的影响。方法将处于对数生长期的SV-HUC-1细胞分别进行急性染毒[暴露于含终浓度为0(对照)、1、2、4、8、10μmol/L亚砷酸钠的培养基染毒24 h]和慢性染毒[以含终浓度为0(对照)、0.5μmol/L亚砷酸钠的培养基染毒40代]。采用逆转录PCR(RT-PCR)方法检测细胞STAT3 m RNA表达情况。结果与对照组比较,4、8、10μmol/L亚砷酸钠暴露组SV-HUC-1细胞内STAT3 m RNA的表达水平均升高,差异均有统计学意义(P<0.05);且随着亚砷酸钠染毒剂量的升高,SV-HUC-1细胞内STAT3 m RNA的表达水平呈先升高后下降的趋势。0.5μmol/L亚砷酸钠慢性暴露组SV-HUC-1细胞内STAT3m RNA的表达水平高于对照组,差异有统计学意义(P<0.05)。结论砷可能通过调控STAT3的表达而在砷诱导的SVHUC-1细胞恶性转化中发挥一定作用。Objective To explore the effects of arsenite （NaAsO2） exposure on the mRNA expression of STAT3 in SV-HU-1 cells. Methods Acute and chronic exposure experiments were used in SV-HUC-1 cells in logarithmic growth phase. The cells were treated with NaAsO2 at 0,1,2,4,8 and 10 μmol/L respectively for 24 hours in the acute trials, while those which were cultured to the 40th generation in chronic trials were exposed to 0,0.5 μmol/L sodium arsenite. 0 μmol/L group was regarded as the control group in both experiments. The mRNA expression of STA T3 was measured with RT-PCR. Results Compared with the control group,the expression of STAT3 mRNA in SV-HUC-1 cells increased significantly （P〈0.05） after exposure to 4,8 and 10 μmol/L NaAsO2 for 24 hours. And with the increase of sodium arsenite exposure dose, the level of STAT3 mRNA expression in SV-HUC-1 cells was increased at first and then decreased.The significant increasing of STA T3 mRNA expression was also found in SV-HUC-1 cells exposed to 0.5 μmol/L NaAsO2 compared with the control group in the chronic trial. Conclusion Arsenic may play an important role in inducing SV-HUC-1 cell malignant transformation through the way of regulating the expression of STAT3.

关 键 词：亚砷酸钠 人膀胱上皮永生化细胞 信号传导与转录激活因子3 

分 类 号：R994.6[医药卫生—毒理学]