男性代谢综合征患者血浆代谢组学研究：基于核磁共振分析(英文)  

Study on Plasma Metabonomics in Men with Metabolic Syndrome: Based on ~1H NMR Analysis

作　　者：张美琳[1] 高玉霞[2] 王璇[1] 刘为桥 张誉文 黄国伟[1] 

机构地区：[1]天津医科大学公共卫生学院营养与食品卫生系,天津300070 [2]天津医科大学总医院心内科,天津300052 [3]天津市和平区健康教育指导中心,天津300070

出　　处：《营养学报》2015年第2期201-208,共8页Acta Nutrimenta Sinica

基　　金：supported by a grant from the National Science and Technology Support Program (No.2012BAI02B02)

摘　　要：目的代谢综合征是以2型糖尿病及心血管疾病危险因素共同聚集的一种代谢性疾病,目前已成为全世界的公共卫生问题之一。通过代谢组学技术识别早期代谢产物的变化不仅可以揭示疾病的发病机制,而且还可以识别高危人群。本研究将通过代谢组学技术分析代谢综合征患者血浆代谢产物的变化,探索代谢综合征发病的特征性生物标志物。方法选取确诊的代谢综合征患者和健康人各5例作为研究对象(年龄,性别和居住地区频数匹配)。采用核磁共振技术检测血浆的代谢谱,并对谱图的积分数据进行偏最小二乘法判别分析,以辨识血浆代谢产物的变化。结果与对照组相比,代谢综合征患者血浆中低密度脂蛋白、极低密度脂蛋白、葡萄糖、饱和脂肪酸及不饱和脂肪酸水平较高,亮氨酸/异亮氨酸、谷氨酰胺、缬氨酸、谷氨酰胺/谷氨酸、磷脂酰胆碱、乳酸/苏氨酸及丙氨酸水平较低。结论代谢综合征患者体内存在脂代谢异常和氨基酸代谢异常,基于核磁共振的代谢组学技术为探讨代谢综合征的发病机制提供了新思路。Objective To analyze the changes of plasma metabolites in patients with metabolic syndrome by metabonomics approach. Methods Five cases with confirmed metabolic syndrome and 5 controls were matched on age, gender and area of residence. Metabolic spectra was acquired with 1H nuclear magnetic resonance（1H NMR） technology, and partial least squares analysis was used to identify the variations of plasma metabolites. Results Compared with the controls, patients with metabolic syndrome had higher levels of low density lipoprotein, very low density lipoprotein, very low density lipoprotein/low density lipoprotein, glucose, glycine/glucose, saturated fatty acids and unsaturated fatty acids, and lower levels of leucine/isoleucine, valine, glutamine/glutamate, phosphatidyl choline, lactate/threonine and alanine in plasma. Conclusion Abnormal lipid and amino acid metabolisms are detected in patients with metabolic syndrome. The 1H NMR-based metabonomics provides new insight into the changes of plasma metabolic profiles in metabolic syndrome.

关 键 词：代谢综合征 代谢组学 核磁共振 脂质代谢 氨基酸代谢 

分 类 号：R589[医药卫生—内分泌]