CD4^+ CD25^+调节性T细胞及TGF-β_1在肺炎支原体肺炎患儿中的表达和意义  

Significance and Expression of CD4^+ CD25^+ regulatory T cells and TGF-β_1 in children with mycoplasma pneumoniae pneumonia

作　　者：刘小群[1] 陈兰举[2] 陈啸洪[1] 张佩红[1] 

机构地区：[1]浙江省绍兴市人民医院(浙江大学绍兴医院)小儿内科,312000 [2]安徽省蚌埠医学院第一附属医院儿内科,233004

出　　处：《中华全科医学》2015年第6期971-973,共3页Chinese Journal of General Practice

摘　　要：目的初步探讨CD4+CD25+调节性T细胞(Treg)及转化生长因子-β1(TGF-β1)在肺炎支原体肺炎(MPP)发病中的作用及可能机制。方法采用流式细胞术(FCM)和ELISA法分别检测2组小儿(MPP组及健康对照组)外周血CD4+CD25+Treg细胞的比例及血清TGF-β1水平,并分析二者之间的相关性。结果 1MPP组患儿外周血中CD4+CD25+Treg细胞比例和血清TGF-β1水平分别为[(5.59%±1.18%)和(467.74±142.05)ng/L],与健康对照组[(8.20%±1.76%)和(730.05±197.21)ng/L]相比,均显著降低(P<0.01);2MPP组小儿血清TGF-β1水平与CD4+CD25+Treg细胞比例呈正相关(r=0.71,P<0.01)。结论 CD4+CD25+Treg细胞比例及TGF-β1水平的降低可能参与MPP的发病。Abstract：Objective To explore the role and possible mechanisms of CIM ~ CD25 ~ Treg and TGF-[31 in Pathogenesis of Mycoplasma pneumoniae pneumonia. Methods MPP and control group of CIM ~ CD25 ~ Tregs＇ ratio were detected by flow cytometry, while serum TGF-[31 levels were determined by Enzyme-linked immuneosorben, and analyze correlation of the two. Results ①The ratio of CD4^＋ CD25^＋ Tregs and serum TGF-β1 levels in MPP group were （5.59% ± 1.18% ） and（467.74 ± 142.05 ） ng/L, which were significantly lower than those of control group [ （8.20% ＋ 1.76% ） and （730.05 ± 197.21 ）ng/L] （P 〈 0.01 ） ;②The serum TGF-β1, levels and CD4^＋ CD25^＋ Tregs＇ ratio of the MPP group were concentration positively correlated （ r = 0.71, P 〈 0.01 ）. Conclusion The reduction of CD4^＋ CD25^＋ Tregs＇ ratio and ser- um TGF-β1 levels could have close ties with the pathogenesis of MPP.

关 键 词：CD4+CD25+调节性T细胞 TGF-β1 小儿 肺炎支原体 肺炎 

分 类 号：R725.6[医药卫生—儿科] R563.1[医药卫生—临床医学]