红景天乙醇提取物对离体大鼠胸主动脉环的舒张作用  

Relaxant effects of ethanol extract from Rhodiola crenulata in isolated rat thoracic aortic rings

作　　者：张洪平[1] 罗婷婷[2] 姜敏[1] 李得新[3] 安冬青[3] 

机构地区：[1] 新疆医科大学附属中医医院国家中医临床研究基地,乌鲁木齐830011 [2] 成都中医药大学药学院,611130 [3] 新疆医科大学中医学院

出　　处：《国际中医中药杂志》2015年第5期418-422,共5页International Journal of Traditional Chinese Medicine

基　　金：新疆名医名方与特色方剂自治区重点实验室开放课题（XJDX-0910-2012-9）

摘　　要：目的：探讨红景天乙醇提取物对离体大鼠胸主动脉环的舒张作用及其可能机制。方法分离Sprague-Dawley大鼠脑主动脉并切成3～4 mm宽的横向环。记录内皮完整或内皮去除的主动脉环的张力。观察不同浓度红景天乙醇提取物（100、300、500、700、900、1100 mg/L）对去氧肾上腺素（1μmol/L）预收缩的主动脉环的舒张作用以及可溶性鸟苷酸环化酶抑制剂ODQ（10μmol/L）预处理对红景天乙醇提取物舒张作用的影响。观察红景天乙醇提取物对无钙高钾液中钙离子和无钙液中去氧肾上腺素引起的收缩作用的影响。结果红景天乙醇提取物对去氧肾上腺素预收缩的主动脉环具有浓度依赖性舒张作用；红景天乙醇提取物对内皮完整和内皮去除的主动脉环最大舒张效应分别为（57.40±21.81）%和（60.51±0.34）%，其半数最大效应浓度为1062.88 mg/L；ODQ预处理对红景天乙醇提取物引起的舒张作用无显著抑制作用（P＞0.05）；红景天乙醇提取物对无钙液中去氧肾上腺素引起的细胞内钙离子释放和无钙高钾液中钙离子内流引起的血管收缩均有显著抑制作用（P 均＜0.05）。结论红景天乙醇提取物具有浓度依赖性的舒张血管作用，其机制可能与抑制细胞外钙离子内流和细胞内钙离子释放有关。Objective To investigate the relaxant effects of ethanol extract fromRhodiola crenulata （Hook.f.et Thoms.） H.Ohba in isolated rat thoracic aorta and its possible mechanisms.MethodsThe thoracic aortas from male Sprague-Dawley rats were isolated and cut into 3- to 4-mm-wide transverse rings, and the tension in endothelium-intact or endothelium-denuded aortic rings was recorded. The relaxant effects of ethanol extract fromRhodiola crenulata in various concentrations （100, 300, 500, 700, 900, and 1 100 mg/L） in aortic rings precontracted with phenylephrine （1 μmol/L） were observed. The effects of pretreatment with soluble guanylyl cyclase inhibitor ODQ （10 μmol/L） on the relaxant effects of ethanol extract fromRhodiola crenulata were observed. The effects of ethanol extract fromRhodiola crenulataon calcium-induced contractions in calcium-free high-potassium medium and phenylephrine-induced contractions in calcium-free medium were also observed.ResultsEthanol extract fromRhodiola crenulatashowed a concentration-dependent relaxation in aortic rings precontracted with phenylephrine. The maximal relaxations of ethanol extract fromRhodiola crenulataln endothelium-intact or endothelium-denuded aortic rings were 57.4% ± 21.81% and 60.51% ± 0.34%, respectively, with a half-maximal effective concentration of 1 062.88 mg/L. The relaxant effects of ethanol extract fromRhodiola crenulatawere not statistically inhibited by pretreatment with ODQ （P〉0.05）. Contractions of aortic rings resulted from phenylephrine-induced Ca2＋ release from intracellular stores in calcium-free medium or high potassium-induced influx of Ca2＋ in calcium-free high-potassium medium were significantly inhibited by ethanol extract fromRhodiola crenulata（allP〈0.05）.ConclusionEthanol extract fromRhodiola crenulatahas a concentration-dependent vasorelaxation, its mechanisms may be involved in blocking extracellular Ca2＋ influx and intracellular Ca2＋ release.

关 键 词：主动脉 胸 血管舒张 血管舒张药 植物提取物 红景天 钙 大鼠 

分 类 号：R285.5[医药卫生—中药学]