PTP-1B及IRS-1在慢性间歇低氧大鼠肝细胞中的表达  

The express of PTP-1B and IRS-1 in liver cells of rats during chronic intermittent hypoxic

作　　者：赵勇[1] 任寿安[1] 蔡月伟 

机构地区：[1]山西医科大学第一医院呼吸科,太原030001

出　　处：《国际呼吸杂志》2015年第9期686-690,共5页International Journal of Respiration

摘　　要：目的探讨慢性间歇低氧（CIH）对大鼠肝细胞蛋白酪氨酸磷酸酶-1B（protein tyrosine phosphatase-1B，PTP-1B）及胰岛素受体底物-1（insulin receptor substrate-1，IRS-1）蛋白表达量的影响及其导致胰岛素抵抗发生的可能机制。方法选取健康雄性SD大鼠24只，按随机数字表法分为正常对照组（NC组）、慢性间歇低氧4周组（CIH4组）、慢性间歇低氧8周组（CIH8组），每组8只。NC组无间歇低氧暴露正常饲养，CIH4组及CIH8组于上午9时至下午5时放人间歇低氧仓暴露于间歇低氧环境中，舱内最低氧浓度为6％～7％。分别于第4周及第8周检测大鼠空腹血糖及空腹胰岛素水平，用稳态模型胰岛素抵抗指数（HOMA—IR）以及胰岛素敏感指数（insulin sensitive index，ISI）评价胰岛素抵抗，SABC法免疫组织化学试剂盒检测CIH大鼠肝细胞PTP1B及IRS-1蛋白表达，以平均灰度值表示PTP-1B及IRS-1的蛋白表达量，并做统计学分析。结果与NC组比较，CIH4组及CIH8组空腹血糖、空腹胰岛素及HOMA—IR升高，ISI降低，差异有统计学意义（P〈0．05），且CIH8组更为显著；与NC组相比较，CIH4组及CIH8组PTP-1B蛋白表达增加，IRS-1蛋白表达减少，差异有统计学意义（P〈0．05），Pearson相关分析显示，HOMA—IR与PTP-1B平均灰度值呈负相关、与IRS-1平均灰度值呈正相关；ISI与PTP-1B平均灰度值呈正相关、与IRS-1平均灰度值呈负相关。结论①CIH暴露使大鼠空腹血糖及胰岛素水平升高且发生胰岛素抵抗，随着CIH暴露时间的延长，胰岛素抵抗程度加重。②CIH导致大鼠肝细胞PTP-1B蛋白表达增加，IRS-1蛋白表达减少，PTP-1B及IRS-1可能共同参与了CIH大鼠胰岛素抵抗的发生发展。Objective To investigate the effects of chronic intermittent hypoxia on the expression of PTP-1B and IRS-1 and the possible mechanism of insulin resistance caused by chronic intermittent hypoxia. Methods According to the principle of random number, twenty-four healthy male SD rats were divided into the normal group （NC group）, the four-week chronically intermittent hypoxia group （CIH4 group） and the eight-week chronically intermittent hypoxia group （CIH8 group） equally. The NC group were raised normally,while the CIH4 and the CIH8 ones were exposed to the intermittent hypoxia cabins from 9 am to 5 pm,in which the lowest air concentration was 6%-7%. The fast blood glucose （FBG） and fasting insulin （FINS） were detected at the fourth week and the eighth week to evaluate the insulin insistence with homeostasis model assessment-insulin resistance index （HMOA-IR） and insulin sensitive index （ISI） ,and the average gray value indicating the expression of PTP-1B and IRS-1 protein was tested with SABC immunohistochemical kits,of which the data were analysed statistically. Results Compared to the NC group,FBG,insulin level and HOMA-IR of the CIH4, CIH8 group increased while ISI decreased （P 〈0.05）,and the trend is more obvious in CIH8 group. Compared to the NC group,PTP-1B protein rised and IRS-1 protein declined in the expression of the CIH4 and CIH8 group （ P 〈 0.05）. Pearson correlation analysis showed that HOMA-IR was negatively correlated with the gray value of PTP-1B and positively corrected with that of IRS-1, ISI had a positive relationship with PTP-1IR and a negative relationship with that of IRS-1. Conclusions ①The exposure of chronically intermittent hypoxia caused increased FNG,insulin level and insulin resistence, which exacerbated as the exposure time extended. ② The chronically intermittent hypoxia induced high expression of PTP-1B protein and low expression of IRS-1 in rats＇ liver cells. PTP-1B and IRS might participate in the insulin resistence of rats caused by

关 键 词：慢性间歇低氧 蛋白酪氨酸磷酸酶-1B 胰岛素受体底物-1 胰岛素抵抗 

分 类 号：R587.1[医药卫生—内分泌]