固定化双孢菇漆酶对萘酚绿B脱色的研究  

The Decoloration of Naphthol Green B by Immobilized Laccase from Agaricus bisporus

作　　者：夏文静[1] 李飞[1] 周惠[1] 潘玥欣 王元爽 

机构地区：[1]南京师范大学泰州学院生物技术与化学工程学院,江苏泰州225300

出　　处：《安徽农业科学》2015年第15期220-221,共2页Journal of Anhui Agricultural Sciences

基　　金：南京师范大学泰州学院院级青年项目(Q201242);泰州市社会发展项目(TS201337;TS019);2013年江苏省大学生创新训练计划项目

摘　　要：[目的]研究固定化双孢菇漆酶对萘酚绿B的脱色效果。[方法]利用固定化的双孢菇漆酶对萘酚绿B进行脱色,研究了温度、萘酚绿B浓度、固定化漆酶用量、酸根离子以及金属离子对脱色效果的影响。[结果]当脱色温度为40℃,脱色时间为4 h,萘酚绿B浓度为60 mg/L时,脱色率可以达到96.6%。酸根离子中PO3-4、CO2-3、Cl-对脱色率有抑制效果,金属离子中Cu2+、Mg2+对脱色率有促进效果,脱色率可以达到99.3%和97.6%。Fe2+对脱色率有抑制,脱色率是不添加金属离子的27%。[结论]该研究可为萘酚绿B的脱色提供了一条新途径。[Objective] The research aimed to study the decoloration effect of naphthol green B by immobilized laccase from Agaricus bisporus.[Method] Using the immobilized laccase from Agaricus bisporus to decolor naphthol green B,the effects of temperature,naphthol green B concentration,immobilized laccase usage,acidic group ion and metal ion on the decoloration were studied.[Result]When decoloration temperature was 40 ℃,and decoloration time was 4 h,and naphthol green B concentration was 60 mg/L,decoloration rate could reach96.6%.In acidic group ions,PO3-4,CO2-3and Cl-had inhibition effects on decoloration rate.In metal ions,Cu2 ＋and Mg2 ＋had promoting effect on decoloration,with decoloration rates of 99.3% and 97.6%,while Fe2 ＋had inhibition effect,which was 27% of decoloration rate not adding metal ions.[Conclusion] The research could provide a new channel for decoloration of naphthol green B.

关 键 词：固定化漆酶 萘酚绿B 金属离子 酸根离子 脱色率 

分 类 号：S188[农业科学—农业基础科学]