大孔树脂纯化皱皮柑果皮黄酮工艺研究  

Purification of flavonoid from rough-peel mandarin peel with macroporous resin

作　　者：张珉[1] 孙化鹏[2] 李良导[3] 彭淼[2] 钟晓红[2] 

机构地区：[1]湖南省林业科学院,湖南长沙410004 [2]湖南农业大学,湖南长沙410128 [3]湖南女子学院,湖南长沙410004

出　　处：《湖南林业科技》2015年第3期50-53,共4页Hunan Forestry Science & Technology

基　　金：湖南省林业科学院青年科研创新基金项目(2013LQJ10)

摘　　要：以皱皮柑果皮为材料,筛选出对皱皮柑果皮黄酮吸附和解吸性能好的大孔树脂,并探讨大孔树脂纯化皱皮柑果皮黄酮的工艺条件。结果表明:弱极性的AB-8大孔树脂对皱皮柑果皮黄酮的吸附和解吸效果较好。AB-8大孔树脂纯化皱皮柑果皮黄酮的最佳工艺条件为:以50%乙醇作为洗脱剂,洗脱剂流速1.5 BV/h,洗脱剂用量为3倍柱床体积。纯化后皱皮柑果皮黄酮纯度可达到15.45%。Used the peel of rough-peel mandarin as the raw materials,the suitable macroporous resin type with good ad-sorption and desorption capacity for flavonoid from rough-peel mandarin peel was screened,and the purification conditions was studied.The results showed that,AB -8 macroporous resin with weak polar had higher flavonoid adsorption capaci-ty.The optimal purification conditions were as follows:50% ethanol as eluent,elution flow rate of 1.5BV /h,and the eluent volume of 3 times bed volume.The purity of flavonoid from rough-peel mandarin peel after purification could reach 15.45%.

关 键 词：皱皮柑 黄酮 大孔树脂 纯化 

分 类 号：R284.2[医药卫生—中药学]