纳米钻石载药体系体内抗肿瘤效应研究  

In Vivo Effect of Anti-cancer Based on Chemotherapeutic Drug-bearing Nanodiamond Particles

作　　者：王青[1] 李林[2] 董宇[1] 王志琴[1] 李英奇[1,2] 

机构地区：[1]山西大学化学化工学院,山西太原030006 [2]山西大学分子科学研究所化学生物学与分子工程教育部重点实验室,山西太原030006

出　　处：《山西大学学报（自然科学版）》2015年第2期318-324,共7页Journal of Shanxi University(Natural Science Edition)

基　　金：国家自然科学基金(21071091);山西省科技攻关计划(社会发展)(20130313021-1);山西省自然科学基金(2009011012-3)

摘　　要：采用荷瘤小鼠为模型,将制备的功能化纳米钻石载药体系,如纳米钻石-聚乙二醇二胺-叶酸-阿霉素(NDPEG-FA-DOX)和纳米钻石-阿霉素(ND-DOX)通过尾部静脉注射至小鼠体内,阿霉素(DOX)作为实验对照组。定期测量小鼠的体重和肿瘤体积,由各组小鼠的平均体重变化表明纳米药物相对DOX具有低毒副作用;通过瘤体变化率对比说明纳米药物注射组的瘤体增长较PBS组缓慢,且ND-PEG-FA-DOX组效果更为显著,说明其具有一定的靶向作用;实验后期采集血液并对动物实施安乐死获取相应器官,绘制器官指数图,同时通过血液测试和生化指标分析,结果均证实所制备的纳米钻石载药体系对小鼠肝脏的毒副作用明显低于传统化疗药物DOX的毒副作用。We explore the anti-tumor efficacy of ND-DOX and ND-PEG-FA-DOX nanomedicine in vivo.The mean body weight of tumor-bearing mice treated with ND-DOX or ND-PEG-FA-DOX showed slight injure.The organ indexes indicate that neither the ND-DOX nor ND-PEG-FA-DOX were toxic to the heart,liver,spleen,which decrease the toxic effects caused by DOX obviously.Excitingly,we found that ND-PEGFA-DOX has stronger anti-cancer effect compared to ND-DOX,which demonstrate ND-PEG-FA-DOX possessing a nice ability of targeting anti-cancer.Furthermore,the analysis of standard hematology markers and the biochemistry results certified that the ND-DOX and ND-PEG-FA-DOX nanomedicine have lower toxicity.

关 键 词：纳米钻石 阿霉素 毒副作用 抗肿瘤 

分 类 号：R962[医药卫生—药理学]