冷诱导基因转录因子CBF1转入黄瓜的研究  

Transformation of Cold-induced Transcription Activator CBF1 into Cucumber(Cucumis satives L.)

作　　者：谭克[1] 赵福顺[1] 吴慧杰[1] 宋述尧[2] 

机构地区：[1]吉林省蔬菜花卉科学研究院,吉林长春130033 [2]吉林农业大学园艺学院,吉林长春130118

出　　处：《北方园艺》2015年第9期79-82,共4页Northern Horticulture

摘　　要：以抗冷性强的黄瓜"山东5号"为试材,研究冷诱导转录因子CBF1基因对黄瓜抗冷性的影响。从"山东5号"黄瓜基因组DNA中扩增并克隆了冷诱导转录因子CBF1基因,将其与CaMV 35S启动子和Nos终止子融合后构建成植物表达载体pROK2-CBF1。通过花粉管通道法转化黄瓜植株,获得了具有卡那霉素抗性的黄瓜再生植株。结果表明:CBF1基因已整合到黄瓜基因组中,转基因植株胁迫期间可溶性糖含量、幼苗含水量显著高于对照;MDA含量、叶片电解质渗透率显著低于对照,黄瓜已具备了较强抗冷性。Taking the strong cold resistance cucumber‘Shandong No.5＇as material,cold induced effect of transcription factor CBF1 gene on cold resistance of cucumber was studied.Cold-induced transcription activator CBF1（CRT/DRE binding factor）was amplified and cloned by PCR from the genomic DNA of cucumber‘Shandong No.5＇and fused with CaMV 35 Spromotor and Nos terminator to construct plasmid pROK2-CBF1.CBF1 gene was introduced into cucumber by pollen tube pathway method.The transgenic nature of the regenerants was demonstrated by plant growth on medium containing kanamycin.The results showed that,PCR analysis confirmed that CBF1 gene was incorporated into the genome of cucumber,seedlings of transgenic plant soluble sugar content,water content was significantly higher than that of the control;the MDA content and the relative electrolyte leakage of cucumber leaves was significantly lower than that of the control,transgenic cucumber has a strong cold resistance.

关 键 词：CBF1转录因子 抗冷 遗传转化 黄瓜 

分 类 号：S642.203.6[农业科学—蔬菜学]