西洛他唑对脂多糖诱导的人THP-1单核细胞分泌TNF-α的影响及机制探讨  

INFLUENCE AND MECHANISM OF CILOATAZOL ON LPS INDUCED TNF-Α SECRETION OF HUMAN THP-1 MONONUCLEAR CELLS

作　　者：陈添华[1] 颜程光[1] 王俊谊[2] 李志樑[3] 

机构地区：[1]井冈山大学临床医学院,江西吉安343000 [2]井冈山大学附属医院,江西吉安343000 [3]南方医科大学附属珠江医院,广东广州510280

出　　处：《井冈山大学学报（自然科学版）》2015年第2期78-80,89,共4页Journal of Jinggangshan University (Natural Science)

摘　　要：目的研究西洛他唑对脂多糖诱导的人THP-1单核细胞分泌TNF-α的影响,探讨西洛他唑对单核细胞的炎症调控作用。方法体外培养人THP-1单核细胞,分为对照组(A组)、LPS组(B组)、西洛他唑组(C组)、西洛他唑+SQ22536组(D组),C组和D组加入LPS刺激24 h。应用ELISA法检测各组细胞培养上清TNF-α水平,并测定各组细胞内c AMP浓度。结果西洛他唑能抑制LPS刺激的人THP-1单核细胞分泌TNF-α,同时升高细胞内c AMP浓度。结论西洛他唑可抑制LPS刺激的人THP-1单核细胞分泌TNF-α,这种作用可能与升高细胞内c AMP浓度有关。Objective：To study the mechanism and influence of ciloatazol on LPS induced TNF-αsecretion of Human THP-1 mononuclear cells and explore the inflammation regulating function of cilostazol on monocytes. Methods：The human THP-1 mononuclear cells were cultured in vitro and divided into 4 groups as control group （A group）, LPS group （B group）, cilostazol group （C group）, cilostazol plus SQ22536 group （D group）. C and D group were stimulated with LPS for 24 h. The TNF-α in supernatant and intracellular cAMP in human THP-1 mononuclear cells on each group was measured by ELISA method. Results：Cilostazol could suppress the LPS induced TNF-αsecretion of human THP-1 mononuclear cells and simultaneously increase the intracellular cAMP concentration. Conclusion： Cilostazol could suppress the LPS induced TNF-α secretion of human THP-1 mononuclear cells which might be related to the increasment of the intracellular cAMP concentration.

关 键 词：西洛他唑 脂多糖 人THP-1单核细胞 肿瘤坏死因子Α 

分 类 号：R541.4[医药卫生—心血管疾病]