逍遥散超临界CO_2提取物的UPLC-PDA指纹图谱及特征成分的含量测定  

UPLC-PDA Fingerprint and Determination of Index Components of Supercritical CO_2 Extract from Xiao-yao-san

作　　者：田俊生[1,2] 左亚妹[1,2] 周玉枝[1] 高晓霞[1] 秦雪梅[1,2] 

机构地区：[1]山西大学中医药现代研究中心,山西太原030006 [2]太原天中益耀科技有限公司,山西太原030006

出　　处：《中国医药工业杂志》2015年第5期473-476,共4页Chinese Journal of Pharmaceuticals

基　　金：国家"重大新药创制"科技重大专项(2012ZX09103201-035);国家国际科技合作计划项目(2011DFA32630);山西省科技计划项目(20130313015-1);山西省科技创新重点团队(2013131015)

摘　　要：利用超高效液相色谱-光电二极管阵列检测器(UPLC-PDA)法,使用BEH C18色谱柱,以乙腈∶0.03%三氟乙酸为流动相,梯度洗脱,建立了14批逍遥散超临界CO2提取物的指纹图谱,并测定不同批次提取物中藁本内酯、白术内酯Ⅰ和白术内酯Ⅲ的含量。结果显示,共确定了15个共有峰。14批逍遥散超临界CO2提取物UPLC指纹图谱相似度均大于0.9。The fingerprints of 14 batches of supercritical CO2 extract from Xiao-yao-san and the quantitative determination of ligustilide, atractylenolide [ and atractylenolide III were established by UPLC-PDA. A BEH Ct8 column was used, with the mobile phase of acetonitrile ： 0.03 % trifluoroacetic acid by gradient elution. Fifteen common peaks were found, and the similarities between UPLC fingerprints of 14 batches of supercritical CO2 extract from Xiao-yao-san were all above 0.9.

关 键 词：逍遥散超临界CO2提取物 超高效液相色谱 指纹图谱 测定 

分 类 号：R932[医药卫生—生药学]