HPLC波长切换法同时测定安神解虑颗粒中4种成分  

Simultaneous determination of four constituents in Anshen Jielü Granules by HPLC wavelength switching

作　　者：王瑞娜[1] 孙耀志 高松 杨玲[1] 赵卫云 冯德文[1] 

机构地区：[1]河南中医学院,河南郑州450008 [2]河南省宛西制药股份有限公司,河南南阳474500

出　　处：《中成药》2015年第5期991-995,共5页Chinese Traditional Patent Medicine

摘　　要：目的建立HPLC法同时测定安神解虑颗粒中紫丁香苷、栀子苷、刺五加苷E、迷迭香酸4种有效成分。方法安神解虑颗粒75%甲醇提取液的分析采用Welch XtimateTMC18色谱柱（4.6 mm×250 mm,5μm）;以乙腈-0.1%磷酸水溶液为流动相,梯度洗脱;体积流量1.0 m L/min;柱温30℃;检测波长分别为265 nm（0~25 min）,238 nm（25~35 min）,220 nm（35~58 min）和330 nm（58~88 min）。结果紫丁香苷、栀子苷、刺五加苷E、迷迭香酸的进样量分别在0.022 8~0.285μg（r=0.999 5）、0.564~7.050μg（r=0.999 7）、0.074~0.925μg（r=0.999 1）、0.053~0.663μg（r=0.999 2）范围内线性关系良好,平均加样回收率分别为97.6%、97.4%、97.9%、97.3%,RSD分别为0.7%、0.8%、0.9%、1.1%。结论本方法简便、准确、重复性好,可为安神解虑颗粒的质量标准研究提供参考。AIM To establish an HPLC method for simultaneously determining four constituents,including syringoside,geniposide,eleutheroside E and rosmarinic acid,in Anshen Jielü Granules. METHODS The analysis was carried out on Welch xtimateTMC18column（4. 6 mm × 250 mm,5 μm）. The mobile phase consisted of acetonitrile-0. 1% phosphoric acid solution in step gradient elution mode. The flow rate was 1. 0 m L/min,the column temperature was 30 ℃ and the detection wavelengths were set at 220 nm,238 nm,265 nm,and 330 nm,respectively. RESULTS The experimental data showed a good linearity in the range of 0. 022 8- 0. 285 μg（r =0. 999 5） for syringoside,0. 564- 7. 050 μg（r = 0. 999 7） for geniposide,0. 074- 0. 925 μg（r = 0. 999 1）for eleutheroside E,0. 053- 0. 663 μg（r = 0. 999 2） for rosmarinic acid,respectively. The average recoveries were 97. 6%, 97. 4%, 97. 9%, and 97. 3%, respectively. The RSDs were 0. 7%, 0. 8%, 0. 9%, and1. 1%,respectively. CONCLUSION This method with strong specificity is simple,accurate and reproducible,and it can provide the reference for quality standard for Anshen Jielü Granules.

关 键 词：安神解虑颗粒 HPLC 紫丁香苷 栀子苷 刺五加苷E 迷迭香酸 

分 类 号：R927.2[医药卫生—药学]