一测多评法测定健脾丸中4种黄酮类成分  

Quantitative analysis of four flavonoids in Jianpi Pills by QAMS

作　　者：刘圆[1] 魏惠珍[1] 龚建平[1] 俞冰荟 金浩鑫[2] 杨世林[1] 饶毅[1] 

机构地区：[1]江西中医药大学,江西南昌330006 [2]中药固体制剂制造技术国家工程研究中心,江西南昌330006

出　　处：《中成药》2015年第5期995-999,共5页Chinese Traditional Patent Medicine

基　　金：十二五国家科技支撑计划(2011BAI04B06);2015年版国家药典委员会课题中药提取物新技术在中药质量控制中的应用(2014年)

摘　　要：目的建立以橙皮苷为内标的一测多评法,测定健脾丸(党参、白术、陈皮、枳实、山楂、麦芽)中4种黄酮类成分(芸香柚皮苷、柚皮苷、橙皮苷、新橙皮苷)。方法来自10个企业的健脾丸(浓缩丸和大蜜丸)以甲醇乙酸提取,HPLC分析采用依利特C18柱(4.6 mm×250 mm,5μm);流动相为甲醇-5.7%醋酸水(31∶69);体积流量为1.0 m L/min,检测波长283 nm。结果 4种黄酮类成分在各自进样量范围内呈良好的线性关系,在岛津LC 10-AT、Waters 2695-2996和Angilent 1260液相色谱系统的相对校正因子重现性良好(RSD<5%)。以橙皮苷为内标的测定结果与外标法测定结果相对误差<3%。结论以橙皮苷为内标建立的一测多评法可用于健脾丸中4种成分的定量分析和对其进行质量评价。AIM To determine the four flavones（narirutin,naringin,hesperidin and neohespridin） in Jianpi Pills（Codonopsis Radix,fried Atractylodis macrocephalae Rhizoma,Citri Reticulatae Pericarpium,fried Aurantii Fructus Immaturus,fried Crataegi Fructus,fried Hordei Fructus Germinatus） using quantitative analysis of multicomponents by single marker（hesperidin）（QAMS）. METHODS The separation of methanol-acetate acid extract of Jianpi Pills,purchased from ten manufacturers, was performed on Elite C18（4. 6 mm × 250 mm,5μm）,using a mixture of methanol-5. 7% acetic acid（31 ∶ 69） as mobile phase,the flow rate was 1. 0 m L /min,and detection wavelength was set at 283 nm. RESULTS There was a good relationship between four flavones and relative correction factors repeated on Shimadzu LC 10-AT,Waters 2695-2996,and Angilent 1260 HPLC systems.The relative error of measured values in internal and external standard method was less than 3%. CONCLUSION The QAMS method established on hesperidin can evaluate the quality of Jianpi Pills.

关 键 词：一测多评 健脾丸 相对校正因子 芸香柚皮苷 柚皮苷 橙皮苷 新橙皮苷 

分 类 号：R927.2[医药卫生—药学]