高效液相色谱法测定黄连上清片中欧前胡素的含量  

Determination of Imperatorin in Huanglianshangqingpian by HPLC

作　　者：曹恒涛[1] 

机构地区：[1]安阳市食品药品检验所,安阳455000

出　　处：《北方药学》2015年第5期10-11,共2页Journal of North Pharmacy

摘　　要：目的:建立黄连上清片中欧前胡素的HPLC测定方法。方法:采用高效液相色谱仪waters Symmetry C_(18)色谱柱(4.6mm×250mm,5μm),乙腈:水流动相,流速1.0ml/min,柱温25℃,PDA检测。结果:欧前胡素在0.012033~0.24066μg的范围内线性关系良好,平均回收率为102.60%(RSD为1.78%)。结论:本文建立的方法简单快速准确,重现性好,可用来测定欧前胡素的含量。Objective To establish a way for determination of Imperatorin in Huanglianshangqingpian by HPLC .Method ：The substance was determined with a HPLC . An waters Symmetry C18 column （4.6mm×250mm,51μm）was eluted with acetontrile- water at a flow rate of 1.0 ml per min and detected by PDA at column temperature 25℃ .Result： Imperatorin had a good linearity relationship between 0.012033ug^0.24066ug and the average recovery was 102.60%（RSD 1.78% ）. Conclusion： The method can be used to determined of Imperatorin in huanglianshangqingpian.

关 键 词：高效液相色谱法 黄连上清片 欧前胡素 含量测定 

分 类 号：R927.2[医药卫生—药学]