六味地黄生物制剂总多糖的精制工艺优选  

Optimization of Purification Technology of Total Polysaccharides from Liuwei Dihuang Biological Preparation

作　　者：陈凯[1] 郭丽冰[1] 王计长 刘文秀[1] 徐文雅[1] 赵越[1] 

机构地区：[1]广东药学院中药学院,广州510006

出　　处：《中国实验方剂学杂志》2015年第10期1-4,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81374072)

摘　　要：目的:优化六味地黄生物制剂总多糖的精制工艺,为该制剂的开发提供参考。方法:以粗多糖质量为指标,采用正交试验考察醇沉浓度、醇沉时间、药液相对密度对总多糖醇沉工艺的影响。在单因素试验基础上,以蛋白质清除率和总多糖保存率的综合评分为指标,采用正交试验优化三氯乙酸(TCA)-正丁醇法脱蛋白工艺。以色素清除率和总多糖保存率的综合评分为指标,利用正交试验优化活性炭除色素工艺。结果:最佳醇沉工艺为醇沉浓度85%,醇沉时间6 h,药液相对密度1.05;粗多糖质量8.92 mg。最佳脱蛋白工艺为粗多糖溶液与TCA-正丁醇体积比1∶2,TCA-正丁醇(1∶15),振荡时间20 min,静置时间0.5 h;蛋白质清除率80.23%,总多糖保存率85.45%。最佳除色素工艺为脱色温度40℃,活性炭加入量6%,脱色时间40 min;色素清除率74.84%,总多糖保存率78.69%。结论:优选的精制工艺稳定可行,适用于六味地黄生物制剂总多糖保健产品的开发。Objective： To optimize purification technology of total polysaccharides from Liuwei Dihuang biological preparation. Method： With the amount of crude polysaccharides as index,orthogonal test was adopted to optimize alcohol precipitation process. Based on single factor tests,taking composite score of deproteinization rate and retention rate of total polysaccharides as index,orthogonal test was adopted to optimize deproteinization process. With composite score of decolorization rate and retention rate of total polysaccharides as index,orthogonal test was adopted to optimize decolorization process. Result： Optimum alcohol precipitation process was as follows：alcohol precipitation concentration of 85%, alcohol sedimentation time for 6 h, relative density of solution concentration of 1. 05. Optimum deproteinization process were as follows： volume ratio of total polysaccharide solution to trichloroacetic acid（TCA） of 1 ∶ 2,volume ratio of TCA-butanol of 1 ∶ 15,vibration time of 20 min,standing for 0. 5 h. Optimum decolorization process was as following： decolorization temperature of 40 ℃,the amount of activated carbon 6%,decolorization time of 40 min. Quality of crude polysaccharides was 8. 92 mg,deproteinization rate was 80. 23%,decolorization rate was 74. 84%,retention rate of total polysaccharides was78. 69%. Conclusion： These optimized extraction processes are stable and feasible, which are suitable for development of health products of total polysaccharides from Liuwei Dihuang biological preparation.

关 键 词：六味地黄生物制剂 总多糖 醇沉工艺 脱蛋白工艺 除色素 

分 类 号：R283.6[医药卫生—中药学] R284.1[医药卫生—中医学]