疱疹康喷雾剂提取工艺考察  

Optimization of Extraction Process for Paozhenkang Spray

作　　者：吴桂芳[1] 冯素香[2] 黄天山 牛德兴 梁生旺[1] 

机构地区：[1]广东药学院中药学院,广州510006 [2]河南中医学院药学院,郑州450008 [3]郑州德兴保健品公司,郑州450008

出　　处：《中国实验方剂学杂志》2015年第10期30-33,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:优选疱疹康喷雾剂的提取工艺。方法:以盐酸小檗碱含量和出膏率的综合评分为指标,通过正交试验考察提取时间、提取次数、加水量对水提工艺的影响。以芦荟大黄素、大黄酸、大黄素、大黄酚、大黄素甲醚总含量和出膏率的综合评分为指标,利用正交试验考察乙醇体积分数、提取时间、提取次数、乙醇用量对醇提工艺的影响。采用HPLC测定盐酸小檗碱及5个大黄苷元的含量。结果:最佳水提工艺为加12倍量水提取3次,每次75 min;盐酸小檗碱质量分数6.07%。最佳醇提工艺为加10倍量75%乙醇回流提取2次,每次75 min;5个大黄苷元总质量分数1.96%。结论:优选的提取工艺稳定可行,适用于疱疹康喷雾剂的工业化生产。Objective： To optimize extraction process of Paozhenkang spray. Method： Taking composite score of the content of berberine hydrochloride and dry extract rate as index, orthogonal test was adopted to investigate influence of extraction time, extraction times, the amount of water on water extraction process. With composite score of the total content of aloe-emodin, rhein, emodin, chrysophanol, physcion and dry extract rate as index, orthogonal test was adopted to optimize alcohol extraction process by taking ethanol concentration, alcohol amount, extraction time and times as factors. Contents of berberine hydrochloride and five aglycones from Rhei Radix et Rhizoma were determined by HPLC. Result： Optimun water extraction process consisted of adding 12-fold of waters, decocting 3 times, 90 rain for each time, the content of berberine hydrochloride was 6.07%. Optimun alcohol extraction process was as following： refluxing extracted twice with 10-fold of 75% ethanol, 75 min for each time; the total content of five aglycones was 1.96%. Conclusion： These optimized extraction processes are stable and feasible for industrial production of Paozhenkang spray.

关 键 词：疱疹康喷雾剂 盐酸小檗碱 芦荟大黄素 大黄苷元 综合评分 

分 类 号：R283.6[医药卫生—中药学] R284.1[医药卫生—中医学]