腹泻型肠易激综合征模型大鼠海马与结肠组织中细胞因子的含量变化及mRNA的表达  

Research on Cytokine and mRNA Expression in Entorhinal Cortex and Colon Tissue of IBS-D Model Rats

作　　者：李得堂[1] 涂星[1,2] 柴玉娜[2] 唐洪梅[1] 黄育生[2] 王婷[2] 

机构地区：[1]广州中医药大学第一附属医院,广州510405 [2]广州中医药大学,广州510405

出　　处：《中国实验方剂学杂志》2015年第10期128-132,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81073160);广东省自然科学基金项目(1015104701000041;S2012010010539)

摘　　要：目的:探讨腹泻型肠易激综合征(IBS-D)模型大鼠海马与结肠组织中细胞因子白细胞介素1β(IL-1β),白细胞介素2(IL-2)和肿瘤坏死因子-α(TNF-α)的含量变化及其mRNA的表达,初步分析脑-肠轴中细胞因子在IBS-D中的发病机制。方法:取健康SD大鼠30只,分为正常组和模型组,正常组10只,模型组20只。模型组复制IBS-D模型,造模成功后取模型大鼠和正常大鼠的海马和结肠组织制成石蜡切片,采用免疫组化染色法分析组织中IL-1β,IL-2和TNF-α的表达,同时采用RTq PCR法检测其海马与结肠组织中IL-1β,IL-2和TNF-αmRNA的表达。结果:与正常组比较,IBS-D模型组海马和结肠组织中IL-1β,IL-2和TNF-α均明显增加,且海马与结肠组织中的IL-1β,IL-2和TNF-α呈一定程度的相关性;IL-1βmRNA在IBS-D组大鼠海马组织中表达量明显增加,TNF-αmRNA在海马和结肠组织中表达量均明显增加(P<0.05),IL-2 mRNA的表达水平无显著变化。结论:IL-1β,IL-2和TNF-α作为致炎介质,在IBS-D脑海马和结肠组织均异常表达,提示其作用机制可能是通过影响神经-内分泌-免疫网络而实现脑-肠轴功能的结构基础改变,进而导致IBS-D的发生和发展。Objective： To detect the expression levels of cytokine interleukin 1β（IL-1β）,interleukin 2（IL-2） and tumor necrosis factor alpha（TNF-α） as well as the mRNA expression in hippocampus and colon tissues of diarrhea predominant irritable bowel syndrome（IBS-D） rat model,and to make a preliminary analysis of the pathogenesis of brain-gut axis in IBS-D. Method： Thirty Sprague Dawley rats were divided into the normal group（n = 10） and the model group（n = 20）. The IBS-D rat model was replicated in the model group,and the expression levels of IL-1β,IL-2 and TNF-α were detected by using immunohistochemical analysis,the mRNA expressions of IL-1β, IL-2 and TNF-α were detected by using fluorescence quantitative PCR method. The relationship of the different expression in hippocampus and colon tissues was analyzed by using SPSS 13. 0. Result：Compared with the normal group,the levels of IL-1β,IL-2 and TNF-α in the hippocampus and colon tissue of increased significantly in the model group, and the results showed some degree of correlation between the hippocampus and colon tissue. The mRNA expression of IL-1β in the hippocampus of IBS-D rats increased significantly,the mRNA expression of TNF-α in the hippocampus and colon tissues increased significantly（P〈0. 05）,but there were no significant changes in the mRNA expression level of IL-2. Conclusion： As the mediators of inflammation,the expressions of IL-1β,IL-2 and TNF-α,were abnormal in the hippocampus and colon tissues of the IBS-D rats,suggesting that the occurrence and development of IBS-D may be contributed by the effect of nerve endocrine immune network and the realization of brain-gut axis function changes.

关 键 词：腹泻型肠易激综合征 脑-肠轴 白细胞介素-1Β 白细胞介素-2 肿瘤坏死因子-α 

分 类 号：R285.5[医药卫生—中药学]