高加速度环境作用颞下颌关节软骨基质成分与相关生长因子及炎性因子的变化  

Changes of cartilage matrix components, related growth factors and inflammatory factors in the temporomandibular joint under high acceleration

作　　者：栗洪师[1] 陈新[1] 冯岩[1] 尹音[1] 曹均凯[2] 

机构地区：[1]解放军空军总医院口腔科,北京市100142 [2]解放军总医院口腔研究所,北京市100138

出　　处：《中国组织工程研究》2015年第11期1663-1667,共5页Chinese Journal of Tissue Engineering Research

基　　金：全军后勤科研面上项目(BWS12J078)~~

摘　　要：背景:强大的加速性能可产生持续性高正加速度(+Gz),使颞下颌骨关节内部受到各种方向的压力和撞击,引起微小创伤,进而引发颞下颌关节紊乱病症。目的:观察颞颌关节紊乱后软骨关节基质成分Ⅱ型胶原、多糖聚糖体、胶原酶以及胰岛素样生长因子1、转移生长因子β1、肿瘤坏死因子α、白细胞介素2与白细胞介素3的变化。方法:建立不同持续性正高加速度(+Gz)环境和作用时间下的大鼠模型,分成阴性对照组,+5 G加速度组,+10 G加速度组。按不同+Gz值进行正高加速度模拟处理,离心5 min,4 d/周,每天连续离心5次,共持续3周;阴性对照组大鼠按相同时间和频次只在离心机的固定装置上固定5 min,不做持续高正加速度离心处理,每天离心前所有动物均空腹12 h。分离并体外培养各组大鼠的颞下颌软骨细胞,提取总蛋白,Western Blot检测。结果与结论:与阴性对照组比较,在持续性高加速度下,颞下颌关节软骨细胞的软骨基质成分Ⅱ型胶原、多糖聚糖体、胶原酶以及胰岛素样生长因子1、转移生长因子β1明显减少,肿瘤坏死因子α、白细胞介素2与白细胞介素3显著增高。结果说明在持续性高正加速度下,颞下颌关节软骨基质成分受到严重影响,修复能力下降,炎症反应增加。BACKGROUND：Under repeated ＋Gz condition from the strong acceleration, smal traumas resulting from pressures and impacts in various directions are formed inside the temporomandibular joint, thereby leading to temporomandibular joint disorders. OBJECTIVE： To investigate the changes in colagen type II, proteoglycans, colagenase, insulin-like growth factor-1, transforming growth factor-β1, tumor necrosis factor-α, interleukin-2 and interleukin-3 in the cartilage matrix after temporomandibular joint disorder. METHODS：Thirty-six rats were randomly divided into three groups： negative control group, only fixed on the centrifugal machine for 5 minutes, five consecutive times per day, 4 days per week, totaly for 3 weeks; ＋5 Gz group, centrifugation at ＋5 Gz for 5 minutes, five consecutive times per day, 4 days per week, totaly for 3 weeks; ＋10 Gz group, centrifugation at ＋10 Gz for 5 minutes, five consecutive times per day, 4 days per week, totaly for 3 weeks. Al animals were on an empty stomach for 12 hours prior to daily centrifugation. Chondrocytes from the temporomandibular tissue were isolated and cultured in vitroto extract total protein that was detected by weste blot assay. RESULTS AND CONCLUSION： Compared with the negative control group, the levels of colagen type II, proteoglycans, colagenase, insulin-like growth factor-1, transforming growth factor-β1 in the cartilage matrix after temporomandibular joint were significantly decreased, but the levels of tumor necrosis factor-α, interleukin-2 and interleukin-3 were significantly increased under repeated ＋Gz conditions. These findings indicate that under repeated ＋Gz stresses, a great damage to the cartilage matrix of the temporomandibular joint is found as wel as reduced repair capability and increased inflammatory reactions.

关 键 词：组织构建 软骨组织工程 高加速度 Ⅱ型胶原 多糖聚糖体 胶原酶 胰岛素样生长因子1 转移生长因子Β1 肿瘤坏死因子α 白细胞介素2 白细胞介素3 

分 类 号：R318[医药卫生—生物医学工程]