生物医用纳米(Y_(0.95)Eu_(0.05))_2O_3的体外细胞相容性  

In vitro cytocompatibility of biomedical(Y_(0.95)Eu_(0.05))_2O_3 nanomaterials

作　　者：帅孟奇 朱文鲁 王友法[1] 

机构地区：[1]武汉理工大学材料复合新技术国家重点实验室,湖北省武汉市430070

出　　处：《中国组织工程研究》2015年第12期1846-1851,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金资助项目(51172172)~~

摘　　要：背景:与有机荧光染料和荧光蛋白相比,稀土纳米材料具有更高的灵敏性和更好的光学稳定性,并且具有更低的细胞毒性。目的:探讨(Y0.95Eu0.05)2O3稀土纳米材料的体外细胞相容性,以及氨基化修饰对材料细胞相容性的影响。方法:采用溶胶-凝胶法合成(Y0.95Eu0.05)2O3稀土纳米材料,并进行氨基化修饰。将10,25,50,100,200 mg/L的氨基化与非氨基化(Y0.95Eu0.05)2O3稀土纳米材料悬液分别与SD大鼠血管平滑肌细胞共培养3 d,CCK-8法检测细胞增殖;将100 mg/L的氨基化与非氨基化(Y0.95Eu0.05)2O3稀土纳米材料悬液分别与L929细胞共培养48 h,荧光染色观察细胞凋亡。结果与结论:随着材料悬液质量浓度的升高,血管平滑肌细胞存活率呈逐渐减少趋势;在材料质量浓度为10,25,50 mg/L时,氨基化组细胞存活率明显高于未氨基化组(P<0.05);当材料质量浓度高达200 mg/L时,未氨基化组细胞存活率降到76%,氨基化组细胞存活率仍然在80%以上,两组比较差异有显著性意义(P<0.05)。氨基化组L929细胞生长状况良好,凋亡细胞数量较少;未氨基化组生长较稀疏,细胞凋亡数量较多,显示出轻微的细胞毒性。表明氨基化修饰可以有效提高(Y0.95Eu0.05)2O3稀土纳米材料的细胞相容性。BACKGROUND：Compared with organic fluorescent dyes and fluorescent proteins, rare earth nanomaterials have higher sensitivity, better optical stability, and lower cytotoxicity. OBJECTIVE： To investigate thein vitro cytocompatibility of （Y0.95Eu0.05）2O3 METHODS：（Y rare earth nanomaterial and the effect of amine functionalization on the material cytocompatibility. 0.95Eu0.05）2O3 nanomaterials were synthesized using sol-gel method, and underwent amine＆nbsp;functionalization. Amine-functionalized samples （10, 25, 50, 100, 200 mg/L） and non-amine-functionalized （Y0.95Eu0.05）2O3 rare earth nanomaterial suspensions were co-cultured with vascular smooth muscle cels of Sprague-Dawley rats for 3 days. Cel proliferation was detected using Cel Counting Kit-8 assay. 100 mg/L amine-functionalized samples and non-amine-functionalized （Y0.95Eu0.05）2O3 rare earth nanomaterial suspensions were co-cultured with L929 cels for 48 hours. Cel apoptosis was observed using fluorescence staining. RESULTS AND CONCLUSION：With increased mass concentration of material suspension, survival rate of vascular smooth muscle cels was gradualy reduced. At the mass concentration of 10, 25, 50 mg/L, cel survival rate was significantly higher in the amine-functionalized group than in the non-amine-functionalized group （P 〈 0.05）. When the mass concentration of materials reached 200 mg/L, the cel survival rate decreased to 76% in the non-amine-functionalized group, but it was stil above 80% in the amine-functionalized group, showing significant differences between the two groups （P 〈 0.05）. The growth of L929 cels was good and the number of apoptotic cels was less in the amine-functionalized group. Cels were sparse and the number of cel apoptosis was more in the non-amine-functionalized group, showing slight cytotoxicity. These data verified that amine-functionalization can improve cytocompatibility of （Y0.95Eu0.05）2O3 rare earth nanomaterial.

关 键 词：生物材料 纳米材料 生物相容性 氨基化修饰 CCK-8 细胞毒性 荧光染色 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]