加入壳聚糖改善丝素蛋白结晶性:力学稳定强度更好的三维支架材料  

Chitosan improves the crystallization of silk fibroin: a three-dimensional scaffold material with better mechanical stability

作　　者：张霞只 司徒方民[1] 彭鹏[1] 焦延鹏[1] 

机构地区：[1]暨南大学材料科学与工程系,广东省广州市510632

出　　处：《中国组织工程研究》2015年第12期1858-1863,共6页Chinese Journal of Tissue Engineering Research

基　　金：国家自然科学基金青年;面上项目(50903039;81101151);广东省优秀博士论文自助项目(sybzzxm201034)~~

摘　　要：背景:丝素蛋白作为天然生物高分子具有良好的生物相容性,但其结晶性能较高、脆性较大,较难得到均匀结构的三维支架材料。目的:通过加入壳聚糖改善丝素蛋白的结晶性,得到具有稳定力学强度的三维组织工程支架材料。方法:采用CaC l2/CH3CH2OH/H2O三元溶液溶解蚕茧,提取丝素蛋白并制成溶液;使壳聚糖溶液与丝素蛋白溶液的质量比分别为2∶1、1∶1、1∶2,采用冷冻干燥法制备多孔丝蛋白/壳聚糖支架材料,并对复合支架进行甲醇浸泡交联处理,以单纯的丝素蛋白和壳聚糖支架为对照。对各组支架进行扫描电镜观察,红外光谱和X射线衍射、孔隙率、吸水率及在水环境下的周期性循环压缩力学性能测试。结果与结论:复合支架的孔隙结构比纯丝素蛋白支架更均匀有序,并且壳聚糖含量越高,复合支架的孔隙越均匀有序、孔隙率越低、支架结构越致密;当壳聚糖与丝素蛋白的混合比例为1∶2时,在复合支架中的吸水率最高,同时高于丝素蛋白支架,但低于壳聚糖支架;随丝素蛋白成分的增加,复合支架弹性更好,保持形状的能力更优。BACKGROUND：Silk fibroin as natural biological macromolecules has good biocompatibility, but it is difficult to make the three-dimensional scaffold with uniform structure because of its higher crystalization performance and bigger brittleness. OBJECTIVE：To improve the crystalization of silk fibroin through the addition of chitosan, and to get three-dimensional tissue engineering scaffolds with better mechanical strength. METHODS： CaCl2/CH3CH2OH/H2 RESULTS AND CONCLUSION：The introduction of chitosan could improve the properties of scaffolds. The porosity of the composite scaffold with lower porosity was more uniform and orderly with higher content of O ternary solution was used to dissolve silkworm cocoon to extract silk fibroin and form solution. Silk fibroin solution and chitosan solution were mixed according to different mixing ratios of 2：1, 1：1, 1：2, respectively, and then porous silk protein/chitosan scaffolds were prepared by freeze-drying method and treated by methanol. Scaffold morphology was observed by scanning electron microscopy, the chemical structure and crystaline state of the scaffolds were characterized through infrared spectrum and X-ray diffraction test, respectively. Also, the porosity and water uptake were tested and periodic cycle compression mechanical properties under the water environment were determined. chitosan. When the mixture rate of chitosan and silk fibroin was 1：2, the water uptake rate was the highest in the composite scaffolds, and also higher than that of the silk fibroin scaffold but lower than that of the chitosan scaffold. With the increase of silk fibroin, the composite scaffolds had better elasticity and stronger ability to maintain the shape.

关 键 词：生物材料 材料相容性 丝素蛋白 壳聚糖 复合支架 性能研究 国家自然科学基金 

分 类 号：R318[医药卫生—生物医学工程]