小鼠骨髓间充质干细胞联合胰岛移植调节Tfh细胞及诱导免疫耐受的机制  

Mechanism of immunotolerence induced by bone mesenchymal stem cell co-transplanted with islets through regulating Tfh in NOD mice

作　　者：关蛟 张正筠[2] 周尊强[2] 李浩[2] 佟大年[2] 周光文[2] 

机构地区：[1]上海交通大学附属第一人民医院普外科,200080 [2]上海交通大学附属第六人民医院普外科

出　　处：《中华肝胆外科杂志》2015年第4期262-265,共4页Chinese Journal of Hepatobiliary Surgery

基　　金：国家自然科学基金面上项目（81170721）;上海市科委医学引导项目（14411960700）;上海市科委浦江人才项目（14PJ1407300）

摘　　要：目的 研究骨髓间充质干细胞（MSC）联合胰岛移植对滤泡辅助性T细胞（Tfh）分化的调节作用及诱导非肥胖糖尿病鼠（NOD）胰岛移植免疫耐受的影响.方法 实验动物分为4组.A组：单纯胰岛移植组;B组：MSC与胰岛联合移植组（MSC：0.5 × 10^6个）;C组：MSC与胰岛联合移植组（MSC：2×10^6个）;D组：MSC与胰岛联合移植组（MSC：3×10^6个）.ELISA法检测各组小鼠糖尿病自身抗体GAD65Ab、IAA表达水平,流式细胞仪检测各组外周血及脾脏Tfh细胞水平.结果 与胰岛单独移植组NOD小鼠相比,MSC与胰岛联合移植组NOD小鼠胰岛移植物生存时间明显延长,体内糖尿病自身抗体GAD65 Ab和IAA浓度明显降低,CI4＋T细胞向Tfh分化明显降低.结论 MSC联合胰岛移植可以通过调节Tfh细胞分化从而保护胰岛移植物.Objective To study the regulation mechanism of bone mesenchymal stem cell （MSC）combined co-translation of islets in differentiation of Follicular Helper T cell （Tfh）,and its roll on immunotolerence induction in non-obese diabetic （NOD） mice transplantation model.Methods The NOD mice were divided into 4 groups：Group A with islet transplantation alone;Group B with MSC co-transplantation with islets （MSC：0.5 × 10^6）;Group C with MSC co-transplantation with islets （MSC：2 × 10^6）;Group D with MSC co-transplantation with islets （MSC：3 × 10^6）.ELISA was used to test the expression level of diabetes autoantibody GAD65Ab and IAA.Tfh cell count was detected by FACS.Results The survival time of transplantation groups was much longer in MSCs co-transplantation group than islet-alone group;the level of GAD65Ab,IAA and Tfh cell count were much lower in MSCs co-transplantation group than islet-alone group.Conclusion MSC may protect the islet transplants by regulating the Tfh cell differentiation.

关 键 词：骨髓间充质干细胞 滤泡辅助性T细胞 糖尿病 非肥胖糖尿病鼠 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学]