蛇根木WRKY转录因子的鉴定及特征分析  

Identification and Characterization of WRKY Transcription Factors in Rauvolfia serpentina

作　　者：杨致荣[1] 陈钊[1] 樊娟[2] 李润植[3] 王兴春[2] 

机构地区：[1]山西农业大学文理学院,山西太谷030801 [2]山西农业大学生命科学学院,山西太谷030801 [3]山西农业大学分子农业与生物能源研究所,山西太谷030801

出　　处：《植物生理学报》2015年第4期445-454,共10页Plant Physiology Journal

基　　金：山西省自然科学基金(2013011028-1);山西农业大学引进人才博士科研启动资金项目(2013YJ42);山西省留学人员科技活动择优资助项目(晋人社厅函[2014]779号)

摘　　要：WRKY是一个转录因子大家族,在植物次生代谢过程起着重要的调控作用。本文对蛇根木Rs WRKY转录因子的数目、类型、保守基序及其表达模式等进行了分析。从蛇根木的34 109个蛋白中鉴定出48个Rs WRKY转录因子;这48个转录因子根据其WRKY域的特点可分为1、2和3三类,第1类Rs WRKY包括13个成员,其中8个含有两个WRKY域;第2类有29个成员,可分为2-a、2-b、2-c、2-d和2-e五个亚类;第3类Rs WRKY包括6个成员,全部属于3-a类。这48个WRKY基因的表达具有组织器官特异性,按照其表达谱可分为五大类群。第I、II和V类群的Rs WRKY在根、茎和叶中的表达量相对较高,表达模式分别与第II、III和I类阿吗灵生物合成关键基因的表达模式相似,是未来研究Rs WRKY介导的次生代谢调控网络的候选靶基因。WRKY is one of the largest transcription factor families involved in plant secondary metabolism reg-ulation. In this study, the gene number, classiifcation, conserved motif and expression pattern of the WRKY transcriptional factors in Rauvolifa serpentina were analyzed. A total of 48 RsWRKYs were identiifed from all of the 34 109 proteins in R. serpentina. According to the structural feature of the WRKY domain, the 48 RsWRKYs were classified into 3 groups. The group 1 included 13 WRKY members, among which only 8 members had two WRKY domains. The group 2 included 29 RsWRKYs, and they were classiifed into 5 sub-groups （2-a, 2-b, 2-c, 2-d and 2-e）. The group 3 had 6 members and all of them belonged to group 3-a. These 48 Rs-WRKY genes displayed different expression patterns, and could be divided into 5 groups. Among these ifve groups, the genes of group I, II and V were highly expressed in roots, stems and leaves, and their expression patterns were similar with the group II, III, and I genes involved ajmaline biosynthesis respectively. Thus, these WRKY genes are the putative targets for second metabolite engineering mediated by RsWRKYs in R. serpentina in the future.

关 键 词：WRKY转录因子 次生代谢 表达谱 蛇根木 

分 类 号：Q943.2[生物学—植物学]