高效甘薯脱毒苗生产及驯化移栽  

Production, Acclimatization and Transplantation of Virus-Free Plantlets of Sweet Potato

作　　者：王鹏[1] 纪瑞瑞[1] 孔祥远[1] 隋炯明[1] 乔利仙[1] 郭宝太[1] 孙世孟[1] 王晶珊[1] 

机构地区：[1]青岛农业大学生命科学学院、山东省高校植物生物技术重点实验室,山东青岛266109

出　　处：《植物生理学报》2015年第4期455-458,共4页Plant Physiology Journal

基　　金：山东省薯类产业创新团队遗传育种岗位(SDAIT-10-011-03)

摘　　要：选用目前主要推广的甘薯品种‘烟薯25’、‘商薯19’和‘徐薯22’为材料,研究了甘薯脱毒快速繁殖及驯化移栽。结果表明,秋天刚收获的薯块在36℃条件下催芽,10~15 d后芽苗可长到10 cm以上；剥取带有1~2个叶原基的茎尖进行培养,3个供试品种植株再生率均达到90%以上；脱毒试管苗炼苗2~3 d后直接栽植于塑料大棚,移栽成活率达95%以上；移栽5~6周后茎蔓可长到30~40 cm以上,即可剪切茎蔓扦插进行再繁殖。The method on production, acclimatization and transplantation of virus-free plantlets was studied by using cultivars‘Yanshu 25’,‘Shangshu 19’ and‘Xushu 22’ of sweet potato （Ipomoea batatas）. The results in-dicated that the seedlings could grow more than 10 cm after a germination acceleration at 36℃for 10–15 d. Then, stem tips with 1–2 leaf primordia were excised and cultured on the induction medium, the plantlet regen-eration rates were above 90%in the three cultivars. The virus-free plantlets in tubes were acclimated for 2–3 d and subsequently transferred to soil in plastic greenhouse, and the transplanting survival rates of the three culti-vars were over 95%. After having been transplanted for 5–6 weeks, the lateral branches reached 30–40 cm in length, and they can be used for propagation in plastic greenhouse.

关 键 词：甘薯 热处理 茎尖培养 脱毒苗 驯化移栽 

分 类 号：S531[农业科学—作物学]