黑蜂王台病毒中国BQCV-JL1株5'UTR的序列分析  

Analysis of the sequence of the 5'UTR region of the Chinese black queen cell virus-JL1

作　　者：杨倩[1,2] 张健[3] 宋战昀[1] 郑言[1] 王向辉[1] 隋佳辰 王振国[1] 牟峻[1] 

机构地区：[1]吉林出入境检验检疫局检验检疫技术中心,长春130062 [2]吉林农业大学,长春130118 [3]长春生物制品研究所有限责任公司,长春130062

出　　处：《应用昆虫学报》2015年第2期308-314,共7页Chinese Journal of Applied Entomology

基　　金：国家自然科学基金项目(31001065);国家质检总局科技计划项目(2013IK033)

摘　　要：蜜蜂黑蜂王台病毒（Black queen cell virus,BQCV）是引起蜜蜂蜜蜂黑蜂王台病的病原体,主要侵染蜜蜂蜂王幼虫。BQCV基因组为单正链RNA,包括5＇端的ORF 1和3＇端的ORF 2两个开放阅读框。分别编码非结构蛋白和结构蛋白。本实验室首次成功分离鉴定得到中国首株BQCV毒株,命名为中国BQCV-JL1株。该株全长为8 358 nt。【目的】本文主要研究该株核苷酸序列位置为1~1 124 nt的片段,该段序列的1~545 nt为该毒株的5＇UTR区域。【方法】通过Blast及DNAStar等软件对核苷酸及氨基酸序列进行分析。【结果】5＇UTR区域的同源性为94%~99%,为高度保守序列。【结论】经Mega5软件作多重序列对比分析得知,该中国BQCV-JL1株的5＇UTR区域与其他毒株差异性很大,经分析推断原因为碱基的缺失与碱基置换。Black queen cell virus （BQCV） is a pathogen that mainly infects the queen bee larvae. The BQCV genome has a single positive-stranded RNA and two open reading frames （ORF）; ORF 1 at the 5 ＇ end and ORF 2 at the 3＇ end, encoding non-structural, and structural, proteins respectively. Our laboratory has successfully isolated and identified the first BQCV strain in China, named Chinese BQCV-JL1 strain, which consists of 8 358 nucleotides. ｜Objeetivesi To characterize BQCV nucleotide sequences between 1 nt and 1 124 nt, and specifically the fragment containing the 5＇UTR region （1-545 nt）. [Metbodsl Nucleotide and amino acid sequences were analyzed using Blast and DNAStar software. [Results] The 5＇UTR region is highly conserved with homology between 94% and 99%. [Conclusion] Mega5 software for multiple sequence alignment analysis shows that the Chinese BQCV-JL1 strain is very different from other BQCV strains. We infer that this is because of base deletion and base replacement.

关 键 词：BQCV 5'UTR RT-PCR 序列分析 进化树分析 

分 类 号：S895[农业科学—特种经济动物饲养]