HIV-1早期感染者外周血中CD56＋T细胞表型和功能特征分析  

Analysis of the phenotypes and the function of CD56＋T cells during primary HIV-1 infection

作　　者：梁华[1] 贾曼雪[1] 李丹[1] 邵一鸣 

机构地区：[1]中国疾病预防控制中心性病艾滋病预防控制中心,北京102206

出　　处：《中华微生物学和免疫学杂志》2015年第3期161-166,共6页Chinese Journal of Microbiology and Immunology

基　　金：国家科技重大专项（2012ZX10001008,2014ZX10001001-002）;传染病预防控制国家重点实验室项目（2012SKLID103,2011SKLID207）

摘　　要：目的探讨HIV-1早期感染者外周血中CD56＋T细胞表型和功能变化特点，了解该变化与疾病进展状态的相关性。方法采集HIV-1早期感染者样品53例，HIV-1阴性健康对照31例，分离外周血单个核细胞（PBMC）后，流式细胞术检测CD56＋T细胞数量及细胞表面分子CD16、CD161、NKB1、NKG2A、NKp46、NKG2D、NKG2C、CD158a表达水平。将PBMC与K562细胞共孵育，检测CD56＋T细胞分泌细胞因子IFN．吖和TNF—d的水平，以及细胞毒效应分子CD107a的水平。结果HIV-1早期感染者外周血中CD56＋T细胞数量较健康对照明显减少（P=0．025），且与血浆病毒载量呈负相关（P=0．021，r=-0．316）。CD56＋T细胞表面分子CD16（P=0．003）、CD161（P=0．023）、NKBl（P=0．023）、NKp46（P=0．021）表达较健康对照显著降低，其中NKB1的表达水平与CD4＋T细胞数量呈正相关（P=0．007，F=0．364），而与病毒载量呈负相关（P=0．030，r=-0．299）。HIV-1早期感染者CD56＋T细胞自发分泌IFN-1和TNF-d以及细胞毒效应分子CD107a水平明显低于健康对照（P均〈0．001），在靶细胞K562存在情况下，其杀伤能力也显著低于健康对照（IFN-γ和TNF-α：P均〈0．001，CD107a：JP=0．016）。结论HIV-1早期感染者CD56＋T细胞数量下降，表型发生改变，分泌细胞因子和细胞毒效应分子能力下降，提示在HIV-1感染早期CD56’T细胞即受到损伤，且与疾病进展相关。Objective To investigate the changes of phenotypes and function of CD56＋ T cells during primary HIV-1 infection and their relationship with disease progression. Methods Peripheral blood mononuclear cells （PBMCs） were collected from 53 subjects with primary HIV-1 infection and 31 HIV-1- negative healthy subjects. The percentages of CD56＋ T cells and the expression of several phenotypic markers on CD56＋ T cells including CD16, CD161, NKB1, NKG2A, NKp46, NKG2D, NKG2C and CD158a were analyzed by flow cytometry. IFN-γ and TNF-α released by CD56＋ T cells with and without K562 stimulation and the levels of cytotoxic molecular CD107a were measured. Results The percentages of CD56＋ T cells in patients with primary HIV-1 infection were significantly lower than those of healthy subjects （ P = 0. 025 ）. The levels of CD56＋ T cells were negatively related to the viral loads in plasma samples （P=0. 021, r= -0.316）. Compared with healthy subjects, the expression of CD16 （P=0. 003） , CD161 （P=0. 023）, NKB1 （P = 0. 023 ） and NKp46 （P = 0. 021 ） on CD56＋T cells were decreased in patients with primary HIV-1 infection. The levels of NKB1 were positively related to the CD4＋ T cell counts （ P = 0. 007, r = 0. 364）, but were negatively related to the viral loads in plasma samples （P=0.030, r=-0. 299）. Spontaneous secretion of IFN-γ and TNF-α by CD56＋T cells and the expression of CD107a were dramatically in- hibited in patients with primary HIV-1 infection as compared with healthy subjects （ all P 〈 0. 001 ）. Moreover, the killing ability of CD56＋ T cells against K562 target cells was weakened in patients with primary HIV-1 infection as the levels of IFN-3,-, TNF-α- and CD107a-producting CD56＋T cells were significantly decreased （P〈0. 001 for IFN-γ and TNF-α, P= 0. 016 for CD107a）. Conclusion Inhibited expression and altered phenotypes of CD56＋ T cells were identified during primary HIV-1 infection. Lower levels of cytokines and cytotoxic molecular were also

关 键 词：人类免疫缺陷病毒 CD56+T细胞 表型 细胞毒效应 

分 类 号：R512.91[医药卫生—内科学]