汉防己甲素对HL-60细胞DNA甲基转移酶表达的影响  

Tetrandrine affects the expression of DNMT in HL-60 cells

作　　者：郭雅芳[1] 马梁明[2] 鹿育晋[2] 白波[2] 

机构地区：[1]山西医科大学,太原030000 [2]山西医科大学附属大医院血液科

出　　处：《中国医师杂志》2015年第4期541-544,共4页Journal of Chinese Physician

摘　　要：目的 探讨汉防己甲素（Tet）对HL-60细胞DNA甲基转移酶（DNMT）表达的影响.方法 以地西他滨（DAC）为对照,用不同浓度的Tet作用于HL-60细胞,用四甲基偶氮唑蓝法、流式细胞术检测细胞毒性;用实时荧光定量聚合酶链反应法、Western Blot检测DNMT mRNA及蛋白的表达.结果 Tet对HL-60细胞的生长增殖均有抑制作用,呈时间-剂量依赖性（P＜0.01）;2、4μmol/LTet单药作用48 h后DNMT mRNA表达水平与对照组相比均降低,且有剂量依赖性,2μmol/L Tet与4 μnol/L DAC联合后DNMT mRNA表达下降均明显大于各单药组,差异有统计学意义（P均＜0.05）;Tet单药作用48 h后DNMT蛋白表达均降低,与DAC联合后下降更明显.结论 Tet可有效抑制HL-60细胞生长增殖,下调DNMT mRNA及蛋白的表达,与DAC联合后作用更强.Objective To investigate the expression of DNA methyltransferase （DNMT） in HL-60 cells induced by tetrandrine （Tet）.Methods HL-60 cells were treated with different concentrations of Tet and decitabine （DAC） alone and in combination with both.Methyl thiazolyl tetrazolium （MTT） assay was used to assess cytotoxic effect.Flow cytometry （FCM） was used to determine apoptosis rate.Real-time quantitative polymerase chain reaction （PCR） assay was used to quantify mRNA levels of DNMT.Western blot was used to quantify the expression of DNMT protein.Results Tet inhibited the growth and proliferation of HL-60 cells in a time-and dose-dependent manners （both P 〈0.01）.Tet treated HL-60 cells after 48 h at the concentration of 2 μmol/L,and 4 μmol/L,the levels of DNMT gene and protein in the drug administration group decreased compared to the control group.After incubation for 48 h with Tet 2 μmol/L combined with DAC 4 μmol/L,the combination group was significantly depressed.Conclusions Tet could potently inhibit the growth and proliferation of HL-60 cells,reduce the expression levels of DNMT mRNA and protein,and have a more obvious effect in the combination group.

关 键 词：粉防己碱/投药和剂量 HL-60细胞/药物作用/代谢 DNA(胞嘧啶-5-)-甲基转移酶/代谢 

分 类 号：R96[医药卫生—药理学]