小黑杨FLC基因的克隆及功能解析  

Isolation and Functional Analysis of FLC Gene from Poplar( Populus simonii × Populus nigra)

作　　者：孙琦[1] 曲春浦[1] 郑美珠[1] 刘关君[1] 

机构地区：[1]东北林业大学林木遗传育种国家重点实验室,哈尔滨150040

出　　处：《植物研究》2015年第3期363-369,377,共8页Bulletin of Botanical Research

基　　金：国家"863计划"资助项目(2013AA102702);中央高校基本科研业务费专项资金资助(DL13EA03)

摘　　要：MADS-box家族蛋白是一类重要的调控开花的转录因子，FLC是其家族成员的编码基因之一。FLC在调节开花过程，其功能的发挥受多个途径的调控。本研究利用RT-PCR方法在小黑杨雄花芽中克隆出FLC基因的全长cDNA序列PnFLC。该基因的开放读码框为726bp，编码241个氨基酸残基组成的分子量为27．559kD的蛋白质，蛋白质的等电点为9．37。实时定量荧光PCR结果显示，春化能够使PnFLC在小黑杨根、茎、叶各组织中表达量下调57．9％～84％；启动子GUS染色结果表明，PnFLC启动子在分裂活跃的组织中活性较高。拟南芥的遗传转化结果显示，PnFLC可以调控AtAP1、AtSOC1和AtFT基因的表达，同时延迟拟南芥的开花时间。MADS-box family protein is an important class of transcription factors regulating flowering. FLC is a member of MADS-box transcription factors,and the FLC gene is controlled by many pathways, and then to regulate the flowering. We cloned a FLC-homolog gene in male flower buds from Populus simonii × Populus nigra. The open reading frame （ORF） of this gene had 726 bp which encoded a protein containing 241 amino acid. The molecular weight of the predicted protein was 27. 559 KD, and the pI was 9.37. By real-time quantitative PCR, vernalization could down-regulate the expression of PnFLC 57.9% - 84% in the root, stem and leaves tissues of poplar. By GUS staining, the promoter of the PnFLC is highly expressed in actively dividing tissues. Furthermore, by transforming Arabopsis, PnFLC down-regulated the expression of AtAP1, AtSOC1 and AtFT, while delayed the flowering in Arabidopsis.

关 键 词：小黑杨 FLC基因 开花调控 春化 

分 类 号：S792.119[农业科学—林木遗传育种]