Rice bicoid-related cDNA sequence and its expression during early embryogenesis  

作　　者：YangZX AnGY 

机构地区：[1]ShanghaiInstituteofPlantPhysiology,ChineseAcademyofSciences,Shanghai200032,Ching [2]DepartmentofBiology,HenanUniversity,Kaifeng475001,China

出　　处：《Cell Research》2001年第1期74-80,共7页细胞研究（英文版）

摘　　要：Bicoid is one of the important Drosophila maternal genes involved in the control of embryo polarity and larvae segmentation. To clone and characterize the rice bicoid-related genes, one cDNA clone, Rb24 (EMBL accession number: AJ2771380), was isolated by screening of rice unmature seed cDNA library. Sequence analysis indicates that Rb24 contains a putative amino acid sequence, which is homologous to unique 8 amino acids sequence within Drosophila bicoid homeodomain (50% identity, 75% similarity) and involves a lys-9 in putative helix 3. Northern blot analysis of rice RNA has shown that this sequence is expressed in a tissue-specific manner. The transcript was detected strongly in young panicles, but less in young leaves and roots. This results are further confirmed with paraffin section in situ hybridization. The signal is intensive in rice globular embryo and located at the apical tip of the embryo, then, along with the development of embryo, the signal is getting reduced and transfers into both sides of embryo. The existence of bicoid-related sequence in rice embryo and the similarity of polar distribution of bicoid and Rb24 mRNA in early embryo development may implicates a conserved maternal regulation mechanism of body axis presents in Drosophila and in rice.

关 键 词：Base Sequence Body Patterning Cloning  Molecular DNA  Complementary Gene Expression Regulation  Plant Genes  Plant Homeodomain Proteins Molecular Sequence Data Oryza sativa Protein Structure  Tertiary Research Support  Non-U.S. Gov't Seeds Sequence Homology  Nucleic Acid TRANS-ACTIVATORS 

分 类 号：Q944.4[生物学—植物学]