紫外分光光度法和蒽酮-硫酸法测定参芎葡萄糖注射液中总糖含量  

UV Spectrophotometry and Anthrone Sulfur Method Assaying Total Sugar in Shenxiong Glucose Injection

作　　者：朱迪[1] 谭丹[1] 侯靖宇[2] 向文英[2] 王爱民[2,3] 兰燕宇[2,3] 郑林[1,3] 

机构地区：[1]贵阳医学院贵州省药物制剂重点实验室,贵州贵阳550004 [2]贵阳医学院民族药与中药开发应用教育部工程研究中心,贵州贵阳550004 [3]贵阳医学院药学院,贵州贵阳550004

出　　处：《贵阳医学院学报》2015年第4期349-351,355,共4页Journal of Guiyang Medical College

基　　金：国家科技支撑计划课题(2013BAI11B01);贵州省科技重大专项项目[黔科合重大专项字(2011)6019号];贵州省中药现代化专项项目[黔科合中药字(2013)5062号]

摘　　要：目的：建立参芎葡萄糖注射液中总糖含量的良好测定方法。方法：采用紫外分光光度法和蒽酮-硫取法,以葡萄糖为对照品,对参芎葡萄糖注射液中总糖进行含量测定。结果：建立了以葡萄糖为对照品,紫外分光光度法测定参芎葡萄糖注射液中总糖的含量测定方法,且浓度在0.008-0.03 g/L范围内与吸光度线性关系良好,A=35.50C-0.041 80,r=0.999 3,平均回收率为103.1%,RSD为2.9%。结论：蒽酮-硫酸法测定参芎葡萄糖注射液中总糖含量,测定方法简便、准确可靠,可用于该制剂的质量控制。Objective：To establish the method for the determination of total sugar in Shenxiong glu-cose injection.Methods：Adopting UV spectrophotometry and anthrone sulfur method,using glucose as reference to measure total sugar content of Shenxiong injection.Results：Established the method of using glucose as reference to test total sugar of Shenxiong glucose injection by UV spectrophotometry, and it showed a good linear relationship between the absorbance and the concentration between 0.008-0.03 g/L,A =35.50C -0.0418 0,r =0.999 3;the average recovery was 103.1%,RSD was 2.9%.Conclusions：The method is practical,accurate and reliable,can be used as quality control method of Shenxiong glucose injection.

关 键 词：参芎葡萄糖注射液 总糖 紫外分光光度法 

分 类 号：R284.1[医药卫生—中药学]