Apoptosis of erythrocytic stage parasites of Plasmodium berghei chloro-quine-resistant strain  

作　　者：陈克强 宋关鸿 

机构地区：[1]DepartmentofEtiologicalBiology,DepartmentofBasicMedicine,SecondMilitaryMedicalUniversity,Shanghai200433,China [2]DepartmentofEtiologicalBiology,DepartmentofBasicMedicine,SecondMilitaryMedicalUniversity,S

出　　处：《Journal of Medical Colleges of PLA(China)》2002年第1期17-20,共4页中国人民解放军军医大学学报（英文版）

基　　金：Supported by the National Natural Sciences Foundation of China (No. 39880041)

摘　　要：Objective: To explore the characteristics of crisis state at erythrocytic stage of Plasmodium berghei chloroquine-resistant (RC) strain. Methods: Agarose electrophoresis, optical and transmission electron microscopes were used. Patterns of genomic DNA structures and ultra-structures of the erythrocytic parasites were observed in ICA mice (infected with the RC strain) during rising and declining of parasitemia. Results: During the declining parasitemia, the erythrocytic stage parasites of the RC strain showed round or oval appearance with intact plasma membrane and shrank nuclei with no metabolic window, mitochondria or other membranaceous structures. Their DNA electrophoretogram revealed a ladder pattern which evidently differed from the parasites of the RC strain in the rising parasitemia and the chloroquine-sensitive (N) strain.Conclusion: The crisis state of the erythrocytic stage parasites of the P. berghei chloroquine-resistant (RC)strain is characterized by apoptosis.Objective: To explore the characteristics of crisis state at erythrocytic stage of Plasmodium berghei chloroquine-resistant (RC) strain. Methods: Agarose electrophoresis, optical and transmission electron microscopes were used. Patterns of genomic DNA structures and ultra structures of the erythrocytic parasites were observed in ICA mice (infected with the RC strain) during rising and declining of parasitemia. Results : During the declining parasitemia, the erythrocytic stage parasites of the RC strain showed round or o-val appearance with intact plasma membrane and shrank nuclei with no metabolic window, mitochondria or other membranaceous structures. Their DNA electrophoretogram revealed a ladder pattern which evidently differed from the parasites of the RC strain in the rising parasitemia and the chloroquine-sensitive (N) strain. Conclusion; The crisis state of the erythrocytic stage parasites of the P. berghei chloroquine-resistant (RC) strain is characterized by apoptosis.

关 键 词：APOPTOSIS Plasmodium berghei chloroquine-resistant strain transmission electron microscopy 

分 类 号：R531.3[医药卫生—内科学]