关于全自动电化学发光免疫分析仪HCV剩余试剂再利用的研究  

Study of recycling of remaining reagent for HCV detection by automatic electrochemiluminescence immunoassay analyzer

作　　者：刘振杰[1] 钟伟国[1] 曹永坚[1] 李涛[1] 吴子安[1] 徐宁[1] 何文军[1] 

机构地区：[1]广东省中医院芳村医院,广州市510370

出　　处：《职业与健康》2015年第8期1114-1116,1119,共4页Occupation and Health

摘　　要：目的探讨Roche E601全自动电化学发光免疫分析仪丙型肝炎病毒（HCV）剩余试剂合并再利用的可行性。方法采用合并剩余试剂分别检测特定血清中HCV的含量,并进行精密度、准确性、回收率和灵敏度比对分析;与新试剂一起分别检测同一组标本中有反应性和无反应性HCV的含量,并做均值比较及相关性分析。结果合并剩余试剂检测HCV低、中、高浓度血清（或质控）批内、批间变异系数（CV）均符合厂商提供的CV值。质控品准确性在允许范围内,回收率均值在80%-120%之间。用合并剩余试剂与新试剂分别检测有反应性和无反应性标本,各组均值比较,差异无统计学意义（P〉0.05）。结论 Roche E601免疫分析仪可以对剩余试剂合并再利用,能够保证检测质量,降低检测成本。[ Objective ] To investigate the feasibility of recycling of combination of remaining reagent for HCV detection in Roche E601 automatic electrochemituminescenee immunoassay analyzer.[nethods ] HCV in specific sera were detected by combination of remaining reagents respectively. The precision, accuracy, recovery rate and detection sensitivity of remaining reagents were analyzed. Reactive and non-reactive HCV content of same sample was analyzed by original reagents and remaining reagents respectively. The mean comparison and correlation analysis were performed. [Results] The within-run and between-ran coefficients of variation （CV） of the HCV measured by remaining reagents in low ,middle, high levels of sera met the CV the reagent manufacturers provided. The accuracy was within the allowance range. The recovery rate was variable from 80%-120%. Comparing the results of the original reagents with the remaining reagents, there was no significant difference in reactive and non-reactive HCV samples （P〉0.05）. [Conclusion] The remaining reagent can be combined and recycled by Roche E601 automatic electrochemiluminescence immunoassay analyzer, which also can ensure the quality of measurement and the reduction of the cost.

关 键 词：剩余试剂再利用 全自动电化学发光免疫分析仪 病毒性肝炎 丙型肝炎 

分 类 号：R115[医药卫生—公共卫生与预防医学]